FastQCFastQC Report
Thu 26 May 2016
ERR580087_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580087_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5225490
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGT53740.10284203012540451No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA123000.039.1122441
CAACGCA125100.035.613595
GGTATCA75650.033.7915421
ATCAACG135900.032.888063
TATCAAC150700.031.7473562
AACGCAG148400.031.2238886
GTGGTAT22700.030.3350451
TCAACGC154250.029.4930084
GTACTAG45250.028.663671
ACGCAGA163950.026.8865667
CGCAGAG170100.025.7210888
CGTTAAC12200.024.7421681
GTACATG232850.023.881151
TGGTATC27800.023.498662
TACATGG234800.023.2984982
TAGGCAT61600.023.1930375
TAGTACT63100.022.6419124
ACATGGG237250.022.245943
CTGTGCG65300.022.2374559
GCAGAGT193450.021.9603619
Produced by FastQC (version 0.11.5)