FastQCFastQC Report
Thu 26 May 2016
ERR580086_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580086_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5554861
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT223560.4024583153385836No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT172340.3102507875534599No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT102420.18437905106896466No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA108100.051.7740821
GTATCAA199950.042.6214261
CAACGCA234950.035.3659825
ATCAACG237750.034.9746133
TATCAAC253200.033.4729542
AACGCAG263150.032.274566
GTGGTAT31500.032.2503471
GTACTAG42800.031.9771961
TCAACGC267650.031.2415224
ACGCAGA280950.029.5427917
CGCAGAG286350.028.9038668
GCATCGT45550.026.827418
TAGGCAT53050.026.5925835
GCAGAGT307650.026.5069
GTACATG251750.025.276651
CTAGGCA58450.024.862324
TACATGG256250.024.5214142
ACATGGG253200.023.8873753
GAGTACT165950.023.36514712-13
AGGGCTA55800.022.5852665
Produced by FastQC (version 0.11.5)