FastQCFastQC Report
Thu 26 May 2016
ERR580086_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580086_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5554861
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT189860.34179073067714927No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT154060.2773426733810261No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT101960.18355094753946138No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA104400.052.5887641
GTATCAA193600.042.3925441
CAACGCA238100.033.2009665
ATCAACG239450.033.053933
TATCAAC253200.032.299012
GTGGTAT29900.031.5991541
AACGCAG267050.030.5336916
TCAACGC269000.029.6492484
GTACTAG40300.029.2765061
ACGCAGA288650.027.4184537
CGCAGAG293800.026.8898498
GCAGAGT315600.024.7646149
CTGTGCG54750.024.1211789
TACCCCG5550.023.7109645
GCTATTG57100.023.5397788
GGGCACC50650.023.48155694
TAGTACT56950.023.2723124
CTATTGA59500.023.1433899
GGGCTAT61300.023.0002826
AGGGCTA60150.022.65935
Produced by FastQC (version 0.11.5)