FastQCFastQC Report
Thu 26 May 2016
ERR580085_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580085_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3703718
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57330.15479040250904633No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45690.12336252382065804No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA57000.049.4127921
GTATCAA104400.042.2013661
CAACGCA119700.035.108125
ATCAACG122400.034.4086233
TATCAAC129000.033.9649852
AACGCAG132000.032.499246
TCAACGC136650.030.8565244
ACGCAGA143000.028.9153797
CGCAGAG146850.028.0285538
GCAGAGT164050.024.7488489
GTACATG172850.024.5099721
GTGGTAT24050.024.243431
CGTTAAC8600.024.057011
TACATGG175100.023.8951132
ACATGGG174900.023.1932533
GTACTAG23500.022.0096051
CATGGGG133850.021.2471734
TAGTACT25900.020.6904164
GAGTACT64050.020.28682112-13
CTGTGCG24800.020.2744719
Produced by FastQC (version 0.11.5)