FastQCFastQC Report
Thu 26 May 2016
ERR580085_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580085_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3703718
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50100.13526947785981547No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41990.1133725623819092No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA55050.050.5536231
GTATCAA100550.041.6565741
CAACGCA117600.034.1696975
ATCAACG119150.033.72613
TATCAAC129000.032.2157332
AACGCAG132100.031.0230356
TCAACGC134000.030.2679124
ACGCAGA147500.027.1786337
CGCAGAG151800.026.2852698
GTGGTAT21100.024.7302441
GTACTAG21350.023.3397311
GCAGAGT168250.023.2947549
TACCCCG4650.023.2464795
GTACATG174150.022.2968981
TACATGG175950.021.6956272
ACATGGG176450.020.9626963
ACGTTAA7400.020.9584278
GTGATCG14100.020.332338
GTATAAG24100.020.2863641
CGTTAAC7850.019.7556559
Produced by FastQC (version 0.11.5)