FastQCFastQC Report
Thu 26 May 2016
ERR580084_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580084_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7577872
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT207390.27367841525958736No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT176310.23266426247368655No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT99140.130828285302259No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA119700.052.769561
GTATCAA214950.040.5889781
CAACGCA251300.033.896065
ATCAACG257550.033.1278233
TATCAAC267450.032.294642
AACGCAG276200.031.350696
TCAACGC275350.031.089534
ACGCAGA297900.028.4218757
GTACTAG38400.027.8032971
CGCAGAG307050.027.4370088
GTACATG281750.027.426811
GTGGTAT46900.026.5750871
TACATGG287350.026.409262
ACATGGG285650.025.8535253
GCAGAGT337500.024.3378359
CTGTGCG52150.023.8784359
CATGGGG224250.023.457784
TAACGCC19050.022.4561714
TAGTACT55900.022.4537454
ATGGGGG165300.022.3528695
Produced by FastQC (version 0.11.5)