FastQCFastQC Report
Thu 26 May 2016
ERR580084_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580084_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7577872
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT184070.24290460435330657No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT164720.21736973123853237No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT96310.12709372763224294No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA117300.053.1499061
GTATCAA214200.040.937031
CAACGCA258150.032.953825
ATCAACG259050.032.803913
TATCAAC273500.031.7688582
TCAACGC279800.030.521954
AACGCAG284450.030.516086
GTGGTAT44250.027.8384481
GTACTAG38550.027.3199941
ACGCAGA310650.027.2464647
CGCAGAG318700.026.5141878
GTACATG278100.025.0048431
TACATGG280950.024.1319942
CTGTGCG49550.023.9013189
GCAGAGT351850.023.7219479
TGGTATC51800.023.6901452
ACATGGG282750.023.355253
TAGTACT54350.023.3490524
CTAGTAC55200.022.6492253
TAGGCAT54800.021.5274475
Produced by FastQC (version 0.11.5)