FastQCFastQC Report
Thu 26 May 2016
ERR580083_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580083_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4294468
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60770.14150763261013938No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47390.11035127051825745No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA55550.042.1551971
GTATCAA95150.040.6227041
CAACGCA107150.034.6186145
ATCAACG109850.033.7720533
TATCAAC118900.032.1521072
TCAACGC116200.032.1284564
AACGCAG121100.031.0016376
ACGCAGA135000.027.1824847
CGCAGAG142100.025.8240178
GTACATG160900.024.9286541
TACATGG163100.023.842532
ACATGGG165400.022.7139053
GCAGAGT163450.021.7942629
CATGGGG134100.020.7922444
GTGGTAT27800.020.6357781
GTACTAG18050.019.7989521
ATGGGGG92950.019.473155
AGAGTAC141800.018.6120710-11
GAGTACT65250.018.44373512-13
CAGAGTA155400.018.08719610-11
Produced by FastQC (version 0.11.5)