FastQCFastQC Report
Thu 26 May 2016
ERR580083_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580083_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4294468
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55690.12967846075462666No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46510.10830212263777492No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA57500.044.454221
GTATCAA100000.038.29461
CAACGCA114200.032.6765675
ATCAACG115000.032.4507643
TATCAAC119700.031.76522
AACGCAG126100.030.4465776
TCAACGC123700.030.2820914
ACGCAGA141250.026.4157837
CGCAGAG147700.025.0713188
GTACATG161300.024.7353211
TACATGG160500.024.1588422
GTACTAG18400.024.0930391
GTGGTAT28800.023.9079271
ACATGGG164500.022.8573743
GCAGAGT166700.021.790919
CATGGGG131850.020.8175664
TGGTATC31950.020.006232
GAGTACT72100.018.9016312-13
GCGTAAT2302.2832046E-618.3884936
CAGAGTA160350.018.33072910-11
Produced by FastQC (version 0.11.5)