FastQCFastQC Report
Thu 26 May 2016
ERR580082_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580082_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5280055
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72030.13641903351385545No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57820.10950643506554382No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA75500.046.3984381
GTATCAA134100.040.9901731
CAACGCA152350.034.9957435
ATCAACG156800.034.004173
TATCAAC165850.032.774522
AACGCAG172300.031.6948366
TCAACGC173100.030.9642544
ACGCAGA191050.027.8203547
CGCAGAG196200.026.994048
GTACTAG26950.026.1713711
GTGGTAT30500.025.900221
GTACATG227000.023.821331
GCAGAGT219600.023.5643549
TACATGG230250.023.0766532
ACATGGG235200.021.8498293
TAGTACT38600.021.0722854
GAGTACT81250.020.68424612-13
CTAGTAC39250.020.2447433
CTGTGCG39650.019.7958379
CATGGGG182650.019.6922174
Produced by FastQC (version 0.11.5)