FastQCFastQC Report
Thu 26 May 2016
ERR580082_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580082_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5280055
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63710.12066162189598405No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55330.10479057509817605No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA72300.045.6135981
GTATCAA126300.041.912641
CAACGCA146950.034.5091445
ATCAACG147900.034.2563553
TATCAAC158750.032.9204062
AACGCAG167050.031.3392186
TCAACGC166900.030.6660584
ACGCAGA184950.027.1118727
GTACTAG28100.026.3602071
CGCAGAG191400.026.1984758
GTACATG222000.024.1849251
TACATGG224050.023.3885882
GTGGTAT31000.023.285481
GCAGAGT212950.023.2382959
ACATGGG230250.021.9227563
TAGGCAT40300.020.2920785
GAGTACT81900.020.22620212-13
CATGGGG178550.019.5575924
TAGTACT38300.019.2658124
AGAGTAC196150.018.92674310-11
Produced by FastQC (version 0.11.5)