FastQCFastQC Report
Thu 26 May 2016
ERR580081_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580081_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18720057
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT524770.2803250011471653No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT395740.21139892896693638No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC333990.17841291829399877No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT261990.13995149694255737No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA287450.045.0578421
GTATCAA519450.040.8682981
CAACGCA564350.035.537325
ATCAACG584700.034.3372343
GTACTAG179150.032.2104261
TATCAAC657600.032.0329742
AACGCAG659750.031.3687886
GGGCACC190450.030.35380294
GCATCGT175100.030.3322418
GTACATG798800.029.6729831
TAGGCAT210200.029.216265
TCAACGC700200.028.8872914
TACATGG811450.028.7821352
ACGCAGA698550.028.7176917
ACATGGG794500.028.5670663
CGCAGAG711950.028.1368928
TAGTACT233550.027.3249534
CTGTGCG233450.026.8581739
GTGGTAT88750.026.813341
TCTAACG82100.026.6944392
Produced by FastQC (version 0.11.5)