FastQCFastQC Report
Thu 26 May 2016
ERR580081_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580081_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18720057
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT451910.24140417948513726No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT353080.18861053681620735No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT243400.13002097162417828No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA284250.044.8185461
GTATCAA505500.040.1561
CAACGCA561250.034.2836955
ATCAACG584200.032.9932823
GGGCACC191100.031.4122894
TATCAAC651050.030.949272
GTACTAG174850.030.7573811
AACGCAG657950.030.2370056
GTACATG779450.029.2088991
TAGGCAT211250.028.5002445
TACATGG792250.028.3640162
TAGTACT232800.028.1837394
TCAACGC694100.028.10784
ACATGGG772500.028.0113563
CTGTGCG235350.027.9175649
ACGCAGA704250.027.3481857
CTAACGC78350.027.2341693
TCTAACG79600.026.7483122
CGCAGAG723550.026.5992818
TAACGCC80600.026.4738454
Produced by FastQC (version 0.11.5)