FastQCFastQC Report
Thu 26 May 2016
ERR580080_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580080_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6289231
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66870.10632460470922439No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA83400.044.714671
GTATCAA145350.038.8247831
CAACGCA163250.032.890685
ATCAACG170350.031.6048953
TATCAAC179500.031.097762
AACGCAG184650.029.6745786
TCAACGC188500.028.7354684
GTACTAG32300.027.662661
ACGCAGA207400.025.6026137
CGCAGAG215600.024.47598
GTACATG271500.024.2147621
GTGGTAT39000.023.8750021
CGTTAAC9200.023.513261
TACATGG276500.023.3346792
ACATGGG283700.022.0602153
GCATCGT35400.021.9022318
GCAGAGT243050.021.3295089
TAGTACT50400.020.7109324
TAGGCAT50200.020.6989575
GTATAAG37850.020.6245771
Produced by FastQC (version 0.11.5)