FastQCFastQC Report
Thu 26 May 2016
ERR580080_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580080_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6289231
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA84750.043.581471
GTATCAA143400.036.974051
CAACGCA160200.031.800545
ATCAACG163850.031.0362513
TATCAAC179900.029.2854922
AACGCAG181900.028.6767796
TCAACGC182900.028.1629664
GTACTAG34050.025.0750221
GTACATG268800.024.7088891
ACGCAGA206450.024.6293247
TACATGG272900.023.9380822
CGCAGAG216600.023.3016098
GTGGTAT38100.023.1524241
ACATGGG278600.022.925923
CGTTAAC10250.021.6298661
CATGGGG224050.020.934724
TAGGCAT48450.020.5641045
GCAGAGT243100.020.5485659
TAGTACT47550.020.3609584
CTAGTAC49800.020.196353
Produced by FastQC (version 0.11.5)