FastQCFastQC Report
Thu 26 May 2016
ERR580079_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580079_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8670917
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT218100.2515304897970999No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT167950.1936934697910267No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT103740.11964132513320103No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA126000.048.073191
GTATCAA218400.039.5776981
CAACGCA249950.033.3939485
ATCAACG254400.032.8134163
TATCAAC271250.031.6421362
GTACTAG52500.030.8146171
AACGCAG279500.030.5068636
TCAACGC284000.029.558514
GTACATG314750.028.8817671
TACATGG321300.027.9133932
ACGCAGA303900.027.4381587
ACATGGG318800.027.1584913
GCATCGT54750.027.1276668
TAGTACT75700.026.9622754
CGCAGAG316200.026.3545958
TAGGCAT70050.026.3148255
CTGTGCG77450.025.6713359
CTAGTAC82050.025.5065423
CATGGGG264500.025.3368054
CTAACGC24850.025.1707083
Produced by FastQC (version 0.11.5)