FastQCFastQC Report
Thu 26 May 2016
ERR580079_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580079_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8670917
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT190650.21987293846775377No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT156260.1802116200627915No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT103970.11990657966164363No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA123250.046.4970861
GTATCAA211400.039.139621
CAACGCA246950.032.5066765
ATCAACG253450.031.7102783
GTACTAG55450.030.5217821
TATCAAC271800.030.469142
AACGCAG279950.029.295356
TCAACGC280100.028.8774594
GTACATG299900.028.5771661
TACATGG309100.027.415772
TAGTACT76650.026.7340984
ACATGGG307400.026.2825953
ACGCAGA307350.026.1637827
CTGTGCG77450.025.7902939
CGCAGAG320050.025.1110278
CTAGTAC82250.025.0284793
GTGGTAT44650.024.6378781
CATGGGG252250.024.4452174
CTAACGC23300.024.205923
TAACGCC24700.023.5947824
Produced by FastQC (version 0.11.5)