FastQCFastQC Report
Thu 26 May 2016
ERR580078_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580078_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13285811
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT165180.12432812720277295No Hit
CAGTAGGATTTATTCAGGATCTTCCGACGACATTGATTGCTGCATTCCGC138490.10423902613096031No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC137800.10371967507290297No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGCACC158500.040.11945794
CAACGCA260500.037.3671155
GTATCAA295900.036.4128381
ATCAACG278150.035.1362653
CTGTGCG210800.033.33339
TAGTACT212150.033.229334
ACTGTGC217200.032.611278
GTACTGT227300.030.9757466
GTGGTAT24500.030.9053861
TATCAAC350650.030.6348882
TATTCTC50500.029.984135
GTACTAG171400.029.9356271
ACGCAGA325800.029.8769847
TACTGTG251900.029.7791617
TATTCGC23850.029.757499
CGCAGAG327250.029.6445128
GGTATCA178450.029.5963121
TATTAAG31700.029.228132
TACATGG513950.028.7618982
GTACATG521050.028.7116851
Produced by FastQC (version 0.11.5)