FastQCFastQC Report
Thu 26 May 2016
ERR580078_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580078_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13285811
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT133360.10037776391670784No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGCACC157600.041.16198794
CAACGCA258800.036.90285
GTATCAA291750.036.38461
ATCAACG274900.034.8446353
TACCCCG6000.032.9001585
CTGTGCG218000.032.769329
ACTGTGC224550.032.462288
TAGTACT220300.032.3859944
TATTCTC48000.032.01895
GTGGTAT24000.030.9492681
GTACTGT234650.030.5842046
TATCAAC348950.030.5495132
TATTAAG28700.030.2980232
TCAAGAC21900.030.2608743
GTACTAG169850.029.9478911
GGTATCA175450.029.6350941
ACGCAGA323200.029.533667
TACTGTG256750.029.5258647
CGCAGAG325600.029.2439088
TACATGG494900.028.7772732
Produced by FastQC (version 0.11.5)