FastQCFastQC Report
Thu 26 May 2016
ERR580077_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580077_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6930797
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT174260.25142851536410604No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT141310.2038870854246633No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78980.11395514830401178No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA100500.050.7230451
GTATCAA181700.041.487831
CAACGCA214800.033.8107155
ATCAACG220900.032.9181863
TATCAAC229500.032.5086022
AACGCAG236750.031.2154716
TCAACGC240300.030.4964524
ACGCAGA259150.027.7929537
GTACATG269000.027.3242841
CGCAGAG265750.026.9086158
GTACTAG39500.026.7872121
TACATGG276650.026.1864132
ACATGGG272600.025.502553
GTGGTAT41850.024.4964491
GCATCGT39400.024.0882458
GCAGAGT297100.023.5822149
TAGGCAT49800.023.5033685
CATGGGG215050.023.432164
TAGTACT49050.022.0417374
ATGGGGG158600.021.8139725
Produced by FastQC (version 0.11.5)