FastQCFastQC Report
Thu 26 May 2016
ERR580077_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580077_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6930797
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT155380.2241877810012326No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT137550.19846202392019271No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78160.11277202318867513No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA105400.049.5588041
GTATCAA188200.040.7956621
CAACGCA225600.032.7511145
ATCAACG225650.032.722793
TATCAAC241750.031.6418742
TCAACGC245250.030.2992844
AACGCAG249500.030.1389126
GTACTAG37250.027.3892961
ACGCAGA277600.026.5294157
GTGGTAT45950.025.887041
GTACATG268600.025.8536511
CGCAGAG284000.025.7657138
TACATGG272950.025.0623042
ACATGGG274100.024.4180183
GCAGAGT312600.022.9718999
CATGGGG213050.022.3038124
CTGTGCG47500.021.9645279
TGGTATC51400.021.8612962
TAGTACT50550.021.199384
GAGTACT158700.021.11452912-13
Produced by FastQC (version 0.11.5)