FastQCFastQC Report
Thu 26 May 2016
ERR580076_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580076_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5461998
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT145590.2665508116260753No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT117040.2142805618017436No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74020.13551817485103435No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA77850.047.9062651
GTATCAA137000.040.8854681
CAACGCA158200.033.767735
ATCAACG164050.032.70933
TATCAAC173350.031.9594442
AACGCAG176500.031.0247486
TCAACGC182900.029.4657044
GTACTAG33500.028.9201241
GTACATG185200.028.2131021
ACGCAGA194300.027.4802477
TACATGG187950.027.3498922
ACATGGG188250.026.5808263
CGCAGAG201100.026.550068
TAGTACT47550.026.2084474
CTGTGCG47350.026.006189
CTAGTAC50800.025.1806553
GCATCGT34350.025.1762078
CATGGGG154300.024.6563344
TAGGCAT44900.024.1933275
GCAGAGT221700.023.6800569
Produced by FastQC (version 0.11.5)