FastQCFastQC Report
Thu 26 May 2016
ERR580076_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580076_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5461998
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT129570.23722088510468148No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT106560.19509344382769822No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69670.12755405622631133No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA76250.046.4251861
GTATCAA135950.041.4608731
CAACGCA159350.033.8008545
ATCAACG164600.032.722763
TATCAAC177650.031.3260362
AACGCAG181100.030.5710286
TCAACGC183400.029.5734234
TAGTACT48250.027.7614824
GTGGTAT26300.027.706041
GTACATG179300.027.6873951
GTACTAG32000.027.325081
ACGCAGA196650.027.2215587
TACATGG182700.026.5497282
CTGTGCG46750.026.4402339
ACATGGG179100.026.2946413
CGCAGAG204000.026.1488658
CTAGTAC51150.025.6362023
TAACGCC14250.024.4068644
CATGGGG145700.024.225654
CTAACGC13950.023.9209963
Produced by FastQC (version 0.11.5)