FastQCFastQC Report
Thu 26 May 2016
ERR580075_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580075_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4260411
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64860.1522388332956609No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52750.12381434561125676No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA53550.048.64331
GTATCAA98200.042.6138951
CAACGCA117100.034.406915
TATCAAC125000.033.1021042
ATCAACG122250.033.0767143
TCAACGC128800.031.575674
AACGCAG130100.031.3549466
ACGCAGA148050.026.854717
CGCAGAG155150.025.4431428
GTACATG181400.025.1942651
TACATGG186100.024.15432
ACATGGG189300.023.3728543
GTACTAG17950.022.5271761
GTGGTAT26750.022.1472341
GCAGAGT173650.021.9229689
CATGGGG147200.021.9113254
GTATAAG23250.021.2343541
TGATCGC13900.019.6111169
GTGATCG13700.019.5522868
ATGGGGG99900.019.1536125
Produced by FastQC (version 0.11.5)