FastQCFastQC Report
Thu 26 May 2016
ERR580075_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580075_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4260411
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56550.13273367287803922No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49990.11733609738590947No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA62400.048.443731
GTATCAA107150.039.9629751
CAACGCA124150.033.199865
ATCAACG127550.032.3156363
TATCAAC132950.031.496152
AACGCAG134750.031.0060316
TCAACGC135600.030.4661564
ACGCAGA156050.026.5329367
GTACTAG17650.025.6501921
CGCAGAG163050.025.422668
GTACATG185900.024.3531951
TACATGG188950.023.8528232
ACATGGG192650.022.9325543
GTGGTAT28500.022.669391
GCAGAGT183450.021.9295259
CATGGGG150750.021.1066744
TAGTACT26000.019.8841864
GAGTACT70100.019.67673712-13
ATGGGGG100200.019.1370855
TGGTATC31000.018.9503142
Produced by FastQC (version 0.11.5)