FastQCFastQC Report
Thu 26 May 2016
ERR580074_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580074_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5928218
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87970.1483919788374854No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69620.11743832632335721No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA72200.046.6260641
GTATCAA134850.043.0594981
CAACGCA153050.036.182835
ATCAACG154600.035.883613
TATCAAC166850.034.096682
TCAACGC165500.033.6049354
AACGCAG170350.033.266926
ACGCAGA191850.028.8777287
CGCAGAG198000.027.838148
GTACATG215200.024.382311
GCAGAGT226850.023.6187959
TACATGG222800.023.1074432
GTACTAG26250.022.747171
ACATGGG226400.022.2816283
CATGGGG177700.020.291974
GTGGTAT36000.019.720931
GAGTACT99700.019.66258812-13
GCATCGT25000.019.3599668
AGAGTAC202250.019.33600610-11
CAGAGTA218450.019.24687810-11
Produced by FastQC (version 0.11.5)