FastQCFastQC Report
Thu 26 May 2016
ERR580074_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580074_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5928218
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79180.13356458888657605No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63730.10750279426296402No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA75000.045.337651
GTATCAA131850.040.8837851
CAACGCA150550.034.7446145
ATCAACG150400.034.6545683
TATCAAC162650.032.6796952
TCAACGC162350.032.3643044
AACGCAG167350.032.04116
ACGCAGA190550.027.3754657
CGCAGAG197050.026.3770478
GTACATG209750.023.6311971
TACATGG213350.022.909212
GCAGAGT224700.022.6502429
GTACTAG25350.021.9527571
ACATGGG218650.021.4085183
GTGGTAT34900.021.0806581
CGTTAAC9300.020.2843931
CATGGGG170750.019.6248174
AGAGTAC201850.018.77670710-11
CAGAGTA216800.018.7608110-11
GAGTACT100350.018.70868312-13
Produced by FastQC (version 0.11.5)