FastQCFastQC Report
Thu 26 May 2016
ERR580073_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580073_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8228985
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT229420.278795015424138No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT196770.23911819015346364No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT110860.1347189234152207No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA130200.051.3137321
GTATCAA220800.038.711971
CAACGCA261950.031.2440625
ATCAACG264550.030.9921883
TATCAAC280250.030.198132
TCAACGC278400.029.5346264
AACGCAG284800.029.322256
GTGGTAT55250.027.571681
GTACTAG34850.026.8473111
GTACATG287850.026.5912441
TACATGG289050.026.0092982
ACGCAGA315500.025.9773187
CGCAGAG323200.025.256498
ACATGGG292900.024.9588493
TGGTATC62700.022.7960282
CATGGGG226250.022.6489894
GCAGAGT358000.022.1221569
GAGTACT205900.021.0843912-13
ATGGGGG150650.020.8758475
CAGAGTA348400.020.01451510-11
Produced by FastQC (version 0.11.5)