FastQCFastQC Report
Thu 26 May 2016
ERR580073_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580073_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8228985
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT208620.2535185080541525No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT191560.23278691114396247No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT106950.12996742611634363No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA127950.052.3060531
GTATCAA214150.037.506491
CAACGCA259000.030.0306115
ATCAACG261550.029.7203983
GTGGTAT51400.029.4545061
TATCAAC276250.028.7846762
TCAACGC278050.028.1423194
AACGCAG282250.027.9722926
GTACTAG37050.026.0960921
GTACATG284350.025.6925961
TGGTATC58400.024.9466422
TACATGG287300.024.7495082
ACGCAGA317800.024.4590517
ACATGGG286250.023.9707263
CGCAGAG327500.023.5911168
CATGGGG220650.021.5122574
TAGTACT53100.021.2415034
CTGTGCG53700.021.0911759
CTAGTAC51800.020.9583023
GCAGAGT362450.020.9013799
Produced by FastQC (version 0.11.5)