##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR580073_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8228985
Sequences flagged as poor quality	0
Sequence length	100
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.512531375376184	34.0	31.0	34.0	31.0	34.0
2	32.71554353301167	34.0	31.0	34.0	31.0	34.0
3	32.79344305524922	34.0	31.0	34.0	31.0	34.0
4	36.27056897053525	37.0	37.0	37.0	35.0	37.0
5	36.2010889556853	37.0	37.0	37.0	35.0	37.0
6	36.231782413019346	37.0	37.0	37.0	35.0	37.0
7	36.19554100536093	37.0	37.0	37.0	35.0	37.0
8	36.18666153845219	37.0	37.0	37.0	35.0	37.0
9	37.97693931876167	39.0	38.0	39.0	35.0	39.0
10-11	37.92551201636654	39.0	38.0	39.0	35.0	39.0
12-13	37.887017293141255	39.0	38.0	39.0	35.0	39.0
14-15	39.28852349348067	41.0	39.0	41.0	36.0	41.0
16-17	39.27483444191477	41.0	39.0	41.0	36.0	41.0
18-19	39.276280367505834	41.0	39.0	41.0	36.0	41.0
20-21	39.26152672534948	41.0	39.0	41.0	36.0	41.0
22-23	39.18849937142916	41.0	39.0	41.0	36.0	41.0
24-25	39.10948750058482	40.5	39.0	41.0	35.0	41.0
26-27	38.973400486208206	40.0	38.5	41.0	35.0	41.0
28-29	38.86009307344709	40.0	38.0	41.0	35.0	41.0
30-31	38.74033650565653	40.0	38.0	41.0	35.0	41.0
32-33	38.585518019537034	40.0	38.0	41.0	34.5	41.0
34-35	38.54768425267515	40.0	38.0	41.0	34.5	41.0
36-37	38.58345670820885	40.0	38.0	41.0	35.0	41.0
38-39	38.48909051359311	40.0	38.0	41.0	34.0	41.0
40-41	38.357283492435585	40.0	38.0	41.0	34.0	41.0
42-43	38.31736745175741	40.0	38.0	41.0	34.0	41.0
44-45	38.215417818844	40.0	38.0	41.0	33.5	41.0
46-47	38.002602690368256	40.0	37.5	41.0	33.0	41.0
48-49	37.77844831409949	40.0	37.0	41.0	33.0	41.0
50-51	37.60125331374404	40.0	37.0	41.0	33.0	41.0
52-53	37.42197622185482	40.0	36.0	41.0	32.5	41.0
54-55	37.21901583245078	39.5	36.0	41.0	32.0	41.0
56-57	37.003312133878	39.0	35.0	41.0	31.5	41.0
58-59	36.77065762059355	39.0	35.0	41.0	31.0	41.0
60-61	36.50915574642559	38.5	35.0	40.5	31.0	41.0
62-63	36.21935384497602	38.0	35.0	40.0	31.0	41.0
64-65	35.91892365826405	37.0	35.0	40.0	30.5	41.0
66-67	35.58756444446065	37.0	35.0	40.0	30.0	41.0
68-69	35.230106932993564	36.0	34.5	39.0	29.5	41.0
70-71	34.89608827820199	36.0	34.0	39.0	29.0	40.5
72-73	34.54486622590757	35.0	34.0	38.5	29.0	40.0
74-75	34.190372202647104	35.0	34.0	37.0	29.0	39.5
76-77	32.968154760277244	34.5	32.5	36.0	27.0	39.0
78-79	33.59275797683432	35.0	33.5	36.0	29.0	39.0
80-81	33.5282970500007	35.0	34.0	36.0	29.0	37.5
82-83	33.32100028132267	35.0	34.0	36.0	29.0	37.0
84-85	33.10396883698293	35.0	33.5	35.0	29.0	37.0
86-87	32.92453704800774	35.0	33.0	35.0	28.5	36.0
88-89	32.75561238718991	35.0	33.0	35.0	27.5	36.0
90-91	32.59410577367683	35.0	33.0	35.0	27.0	36.0
92-93	32.4742068432498	35.0	33.0	35.0	27.0	35.5
94-95	32.369181800671655	35.0	33.0	35.0	27.0	35.0
96-97	32.267856363816435	35.0	33.0	35.0	26.5	35.0
98-99	32.18600690121564	35.0	33.0	35.0	26.0	35.0
100	30.92039492112332	34.0	30.0	35.0	23.0	35.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.15496457614144532
1101	2	0.09654587531080949
1101	3	0.036204540670752294
1101	4	0.03705793796782331
1101	5	0.03267375660978189
1101	6	0.07017025614764094
1101	7	0.011190034233678148
1101	8	-0.0014192319494057415
1101	9	-0.04371488553513814
1101	10-11	0.011922528675775368
1101	12-13	-0.01804598378165423
1101	14-15	0.035696776854720724
1101	16-17	0.03027187855314395
1101	18-19	-0.001151004867416816
1101	20-21	-0.023347391079745705
1101	22-23	-4.293532780010878E-4
1101	24-25	-0.07158911883977481
1101	26-27	-0.05577964418818482
1101	28-29	-0.09310025022679014
1101	30-31	-0.0594623123531548
1101	32-33	-0.05020028471388116
1101	34-35	-0.1054301528866759
1101	36-37	-0.04306313761460956
1101	38-39	0.03772630964201795
1101	40-41	-9.498342777192192E-6
1101	42-43	-0.03596892964630172
1101	44-45	-0.08047520971103239
1101	46-47	-0.020885722305557408
1101	48-49	-0.11982879930313572
1101	50-51	-0.1024494238259166
1101	52-53	-0.08547836494039274
1101	54-55	-0.04541019510853772
1101	56-57	-0.14586274141491629
1101	58-59	-0.14392782783028935
1101	60-61	-0.1936994354120074
1101	62-63	-0.16908071062007224
1101	64-65	-0.09982869368737823
1101	66-67	-0.15569805715202278
1101	68-69	-0.14984473365711182
1101	70-71	-0.19495198979785755
1101	72-73	-0.14926878571716884
1101	74-75	-0.19962021124239016
1101	76-77	-0.11888656900276118
1101	78-79	-0.07033374255965441
1101	80-81	-0.07848552334560566
1101	82-83	-0.07717744794472736
1101	84-85	-0.08050193720801957
1101	86-87	-0.1554935785797369
1101	88-89	-0.04400021606807769
1101	90-91	-0.022372253141149656
1101	92-93	-0.1436473411774486
1101	94-95	-0.11429516061079426
1101	96-97	-0.127324957078379
1101	98-99	-0.1700718533446235
1101	100	-0.0372292445623863
1102	1	0.12648058422118424
1102	2	0.025728459223188338
1102	3	0.03439414704725152
1102	4	0.02513622791467185
1102	5	0.02290279678279461
1102	6	0.0508890855761166
1102	7	0.033134982993288986
1102	8	0.021533159966232063
1102	9	0.03433326566420902
1102	10-11	0.043839105197484685
1102	12-13	0.0012401264512149623
1102	14-15	0.07349282012715008
1102	16-17	0.00836298622434839
1102	18-19	-0.023062041845413717
1102	20-21	-0.0010505495177923763
1102	22-23	0.010319086555519164
1102	24-25	-0.006585428649522385
1102	26-27	-0.016471731621855668
1102	28-29	-0.03233558693501237
1102	30-31	0.03725100732441433
1102	32-33	-0.011583235660133084
1102	34-35	0.007824915771053043
1102	36-37	0.04695982112608732
1102	38-39	0.04815131779400872
1102	40-41	0.05713196206824733
1102	42-43	0.057424968580512825
1102	44-45	0.0231855113686521
1102	46-47	-0.006335918859178946
1102	48-49	-0.012874771941497443
1102	50-51	0.0012137206299485115
1102	52-53	0.064107765766515
1102	54-55	-0.02946444181900887
1102	56-57	-0.05155973645632628
1102	58-59	0.025030802986016454
1102	60-61	0.03203683969250193
1102	62-63	0.022179445919810803
1102	64-65	1.8254675201490045E-4
1102	66-67	-0.04336784329998977
1102	68-69	0.04659980650423989
1102	70-71	0.0366389143871686
1102	72-73	0.014036549801510034
1102	74-75	0.015572496095565214
1102	76-77	0.0100679202404379
1102	78-79	0.009488258060173393
1102	80-81	-0.00470008309495995
1102	82-83	0.05741722094357016
1102	84-85	-0.015428387199150961
1102	86-87	-0.01831542809857467
1102	88-89	0.023040263728816512
1102	90-91	-7.626826107909324E-4
1102	92-93	-5.387633641689149E-4
1102	94-95	-0.0639655783540789
1102	96-97	-0.05138928534343279
1102	98-99	0.010975654026388781
1102	100	0.1609644621975761
1103	1	0.11002769098602272
1103	2	0.07189182373723924
1103	3	0.027815301328423914
1103	4	0.00958949631306183
1103	5	-0.021185400857255843
1103	6	0.03740469552899839
1103	7	0.017303438766880674
1103	8	0.01440038115344322
1103	9	-0.008471856834788127
1103	10-11	-0.012998516238667435
1103	12-13	-0.014602658790529688
1103	14-15	0.010922688262347435
1103	16-17	0.010002243121114418
1103	18-19	0.020189311240891072
1103	20-21	-0.041690758083433366
1103	22-23	-0.07047612610578113
1103	24-25	-0.03669650255546486
1103	26-27	-0.07337515449141563
1103	28-29	-0.0717638028069345
1103	30-31	-0.10060993639699944
1103	32-33	-0.1268943103144764
1103	34-35	-0.10268546072721563
1103	36-37	-0.11533472932624278
1103	38-39	-0.11952464619322001
1103	40-41	-0.07038051802383904
1103	42-43	-0.1042726160587577
1103	44-45	-0.05276396363217373
1103	46-47	-0.05790277154628143
1103	48-49	-0.11404389487312017
1103	50-51	-0.1137909646856059
1103	52-53	-0.1386239608993094
1103	54-55	-0.09122215759158081
1103	56-57	-0.08235014490382753
1103	58-59	-0.13933876345419804
1103	60-61	-0.15203768344098734
1103	62-63	-0.12779098341998463
1103	64-65	-0.07418719592318723
1103	66-67	-0.09299822362714849
1103	68-69	-0.08227004505038593
1103	70-71	-0.11499726745063299
1103	72-73	-0.10739496347514432
1103	74-75	-0.16689266067994168
1103	76-77	-0.12413097227964442
1103	78-79	-0.023662054443079228
1103	80-81	-0.024525326196624064
1103	82-83	-0.024723624240756692
1103	84-85	0.05920922359048575
1103	86-87	0.04967590596883298
1103	88-89	0.037681633393269465
1103	90-91	-0.01833243484613689
1103	92-93	0.05736269569246133
1103	94-95	0.037735750071391294
1103	96-97	0.07613953571456733
1103	98-99	0.03059247814170618
1103	100	0.09827677496300069
1104	1	0.08708102742424728
1104	2	0.038879894371824264
1104	3	0.0012809718916457769
1104	4	0.01619407709887355
1104	5	-0.016826215792697496
1104	6	0.0014083035530561006
1104	7	8.986463030922209E-4
1104	8	-0.0405628704097154
1104	9	-0.04355224838600691
1104	10-11	-0.049605079222175164
1104	12-13	-0.05925324547744282
1104	14-15	-0.03135672978468307
1104	16-17	-0.020108379891162542
1104	18-19	-0.05429334340971792
1104	20-21	-0.09585127555723716
1104	22-23	-0.08035225377533806
1104	24-25	-0.06931823830777262
1104	26-27	-0.05785390278659719
1104	28-29	-0.12600948731388684
1104	30-31	-0.1380075591857235
1104	32-33	-0.15714464909704162
1104	34-35	-0.09952234105916347
1104	36-37	-0.06688419629537634
1104	38-39	0.005045298221574512
1104	40-41	0.00667602534392131
1104	42-43	-0.09454661894672967
1104	44-45	-0.13288162951543114
1104	46-47	-0.10338493553814487
1104	48-49	-0.1575053397137509
1104	50-51	-0.11179598788332612
1104	52-53	-0.11182887877657066
1104	54-55	-0.169932214157285
1104	56-57	-0.12137288696911241
1104	58-59	-0.10942712622136952
1104	60-61	-0.1915280450341541
1104	62-63	-0.17761002980612517
1104	64-65	-0.14198859515206408
1104	66-67	-0.12200313357325854
1104	68-69	-0.11679467438707292
1104	70-71	-0.1136219483928187
1104	72-73	-0.15749306576243072
1104	74-75	-0.2172937051166386
1104	76-77	-0.2537633724205435
1104	78-79	-0.09482025597459653
1104	80-81	-0.11348099231817343
1104	82-83	-0.06806352394767856
1104	84-85	-0.09382831908067857
1104	86-87	-0.14196439915762227
1104	88-89	-0.07114132211770396
1104	90-91	-0.09282478246704784
1104	92-93	-0.05764401396425001
1104	94-95	-0.049386059100008595
1104	96-97	-0.13574287329436885
1104	98-99	-0.1612829919968135
1104	100	-0.16904553521417043
1105	1	0.12497118847869615
1105	2	0.10347771291195329
1105	3	0.01744830381817053
1105	4	0.023404938280350507
1105	5	-0.01910856245817172
1105	6	0.01650477600709621
1105	7	0.026417990585855478
1105	8	-0.009049202305213555
1105	9	0.036657144676134124
1105	10-11	0.031672558581142596
1105	12-13	0.028324893713303823
1105	14-15	0.05432108992624762
1105	16-17	0.04034725601500355
1105	18-19	0.014688094582830047
1105	20-21	-0.0149885283767901
1105	22-23	-0.02652581339419413
1105	24-25	-0.010850500438984056
1105	26-27	-7.011730586583553E-4
1105	28-29	0.005454630885232348
1105	30-31	0.008123236876095064
1105	32-33	0.004410372253126127
1105	34-35	-0.011609815087219033
1105	36-37	-0.03462713019692387
1105	38-39	0.024903054447726447
1105	40-41	-0.036140528558846086
1105	42-43	-0.01853361563392042
1105	44-45	0.016660853017754107
1105	46-47	0.0032995085123843637
1105	48-49	-0.0796021172546375
1105	50-51	-0.05398458492653191
1105	52-53	-0.08792166575936022
1105	54-55	-0.019075724551804285
1105	56-57	-0.07800431099227723
1105	58-59	-0.08082735951217757
1105	60-61	-0.06231087750922626
1105	62-63	-0.05219365065406123
1105	64-65	-0.02418133064257688
1105	66-67	-0.048017047559625325
1105	68-69	-0.08845074793052987
1105	70-71	-0.09561126077570492
1105	72-73	-0.09143252170378702
1105	74-75	-0.10628018490880464
1105	76-77	-0.07376481336161334
1105	78-79	-0.013001458438132829
1105	80-81	-0.06641762170509935
1105	82-83	-0.021007631466147814
1105	84-85	-0.059156145202187815
1105	86-87	-0.08668894939133764
1105	88-89	-0.06925920863844937
1105	90-91	-0.02441479773752775
1105	92-93	0.018366576325092865
1105	94-95	0.03268006213569663
1105	96-97	-0.06048522850358751
1105	98-99	-0.10239374449960081
1105	100	0.011523984044170987
1106	1	0.1304825065500168
1106	2	0.08140482565467266
1106	3	0.039524537890002875
1106	4	0.022916618528029176
1106	5	-0.011770709094207632
1106	6	0.012955711810960224
1106	7	0.013255824454248
1106	8	0.01665467856788183
1106	9	0.02613006220783376
1106	10-11	-0.022369971657475674
1106	12-13	0.017503434142568608
1106	14-15	0.024968093111958467
1106	16-17	-0.01632543521800045
1106	18-19	0.008886158947383649
1106	20-21	0.0462867682507877
1106	22-23	0.023865207105387753
1106	24-25	5.284213710581298E-4
1106	26-27	0.016650889709204364
1106	28-29	0.03069329703286172
1106	30-31	-0.01666556787017015
1106	32-33	-0.05962023071888467
1106	34-35	0.04851283410949492
1106	36-37	0.046401396039151166
1106	38-39	0.022507928382225373
1106	40-41	0.013894086208246392
1106	42-43	0.03035145088259128
1106	44-45	0.019667463111552763
1106	46-47	-0.031731599303341795
1106	48-49	-0.0026663174720695793
1106	50-51	0.009082360579313331
1106	52-53	0.05609507329605634
1106	54-55	0.02281238342369818
1106	56-57	0.02136533674963914
1106	58-59	-0.05433270740663687
1106	60-61	-0.06640475958928249
1106	62-63	-0.07564675372295682
1106	64-65	0.00640165053232522
1106	66-67	0.02061672361208622
1106	68-69	0.0075855100474626624
1106	70-71	0.018345793320314385
1106	72-73	-0.02034472503306972
1106	74-75	-0.04881497399541246
1106	76-77	-0.04522319395181995
1106	78-79	0.023914348705027066
1106	80-81	0.03768791119167503
1106	82-83	0.07668725076815264
1106	84-85	0.10483544862024274
1106	86-87	0.143074814128326
1106	88-89	0.11178661147383906
1106	90-91	0.07726720619454142
1106	92-93	0.015381833086848928
1106	94-95	0.0372135550595587
1106	96-97	0.04830468162199253
1106	98-99	0.06694042340620854
1106	100	0.1039243707618347
1107	1	0.07255686809759254
1107	2	0.029891069861555764
1107	3	-0.015293669628931639
1107	4	0.007147068553457814
1107	5	-0.01719034956595067
1107	6	-3.111016047796511E-5
1107	7	-0.003391842442511006
1107	8	0.013290472983058521
1107	9	-0.04524819624119658
1107	10-11	-0.025676812386322467
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2316	10-11	0.03294513172110669
2316	12-13	0.032711915379373124
2316	14-15	0.08018204320830336
2316	16-17	0.04065522773212393
2316	18-19	0.04504245000745044
2316	20-21	0.05848920075128916
2316	22-23	0.018571095709411622
2316	24-25	0.01185275138818298
2316	26-27	0.06693901993659068
2316	28-29	0.07956908793970996
2316	30-31	-0.2722408148094786
2316	32-33	-0.10777803554911713
2316	34-35	-0.14593958046813071
2316	36-37	-0.39265155070545177
2316	38-39	-0.22537066801150019
2316	40-41	-0.17094634776034923
2316	42-43	-0.32103459096369846
2316	44-45	-0.15645864136650545
2316	46-47	-0.25723307532442163
2316	48-49	-0.2577527692290573
2316	50-51	-0.23015843546330927
2316	52-53	-0.2290051552921213
2316	54-55	-0.0593750350906177
2316	56-57	-0.8582816870678158
2316	58-59	-0.6813294438551978
2316	60-61	-0.28795507182816493
2316	62-63	-0.11251365551251524
2316	64-65	-0.03166513299790097
2316	66-67	-0.041764636395576815
2316	68-69	-0.05522818011944253
2316	70-71	-0.08408437562430038
2316	72-73	0.019409070019207775
2316	74-75	0.027449009821332027
2316	76-77	0.050973815368713815
2316	78-79	-0.01473710952005547
2316	80-81	-0.012109441233178586
2316	82-83	-0.22419269158677224
2316	84-85	-0.025995516671535768
2316	86-87	-0.1385423869575817
2316	88-89	-0.13534850234758977
2316	90-91	-0.0643543281060559
2316	92-93	-0.04635854581248111
2316	94-95	0.038462129952158364
2316	96-97	-0.16767871068240225
2316	98-99	-2.721061824075832E-4
2316	100	-0.3208734971442695
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	3.0
12	16.0
13	87.0
14	338.0
15	1013.0
16	2583.0
17	5495.0
18	10258.0
19	16357.0
20	23881.0
21	35264.0
22	46508.0
23	51240.0
24	50405.0
25	50963.0
26	55969.0
27	66610.0
28	82007.0
29	100670.0
30	123273.0
31	152776.0
32	192828.0
33	251735.0
34	364692.0
35	558649.0
36	1004582.0
37	2054768.0
38	2401816.0
39	524060.0
40	139.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.706177511300325	18.872363790909375	13.28860967428599	24.13284902350431
2	16.127044780124198	19.67605552160357	39.71391944601377	24.482980252258464
3	19.16596769830427	24.56132696966287	29.726044910104687	26.54666042192817
4	12.991436746814452	16.18597763341363	37.40820441950681	33.41438120026511
5	13.660362518400754	37.350738636922934	34.52419566237428	14.464703182302028
6	32.16131758655533	36.32321604669349	17.19316537823316	14.322300988518025
7	28.077472074904076	31.942265491534915	21.607033096941077	18.373229336619932
8	26.379129318893273	35.25989918531625	19.479837063249793	18.881134432540687
9	26.318216143576883	15.557302010248478	20.109885676217022	38.01459616995761
10-11	25.655174207287406	25.62302561236207	27.562064074683416	21.159736105667104
12-13	25.925281860605065	23.246134180194165	27.83595024364505	22.992633715555723
14-15	23.263056104008314	24.759819211607304	25.38746250664194	26.58966217774244
16-17	22.78570731123123	27.586188877802726	26.23122085717464	23.396882953791398
18-19	22.46712395330045	26.9197413530834	28.267296673802694	22.345838019813456
20-21	23.435880625363996	26.119461524182714	28.09359132843823	22.351066522015063
22-23	23.406177174235474	25.910833587617798	27.865615794340314	22.817373443806414
24-25	23.04781816955554	25.96086273094434	28.16466429334845	22.82665480615167
26-27	22.99653989961486	26.178853983399176	27.807212648977387	23.017393468008578
28-29	22.7329586669667	26.352032074743516	28.048823099425423	22.866186158864366
30-31	23.192311279667965	26.146364345910992	27.974197554831864	22.687126819589174
32-33	23.095521147831093	26.314240188004216	27.683398699340128	22.906839964824563
34-35	23.24002832967059	26.29621155980602	27.893264836540776	22.570495273982612
36-37	23.150532627886538	26.138909965353573	27.681312186458467	23.029245220301416
38-39	23.114089832389197	26.25067604603311	27.866234645399423	22.768999476178266
40-41	23.09246359674718	25.87854901216542	28.09115068109057	22.937836709996834
42-43	22.986169588699514	26.004972254228342	27.770442127634805	23.23841602943734
44-45	22.990461153999366	26.33945964443755	27.718037623112167	22.952041578450913
46-47	23.009882228953295	26.230441776322028	27.353142270749448	23.406533723975233
48-49	23.234732975188752	26.288119706921346	27.3483275688439	23.128819749046
50-51	23.102977300051272	26.40277677012533	27.226734682295383	23.26751124752801
52-53	23.012916555088665	26.560565329353043	27.06336968178531	23.363148433772977
54-55	22.88391887905291	26.45706381206229	27.168559814864718	23.490457494020077
56-57	23.18153567102175	26.401641177549628	26.961838903400963	23.454984248027657
58-59	22.86300127422221	26.5026674451386	27.23764654299706	23.396684737642136
60-61	22.947299613936305	26.6358308280531	27.047505314467102	23.369364243543497
62-63	22.98209737069449	26.459152574772116	26.93428273489188	23.62446731964151
64-65	22.687290021194062	26.424368195939323	27.065002356617434	23.823339426249177
66-67	22.888469984793797	26.365766224050684	26.960689299235046	23.785074491920465
68-69	22.715945592278466	26.398278637843138	27.070919663932862	23.814856105945534
70-71	22.734883093533377	26.196144506424456	27.014473063404832	24.054499336637335
72-73	22.869333337870252	26.365529496088485	26.933827176937157	23.831309989104106
74-75	22.908035180308868	26.56403413903122	26.743505048944787	23.78442563171512
76-77	22.83697959254605	26.48653621365602	26.602016397639254	24.074467796158668
78-79	22.707077185866726	26.517682149079143	26.655307464067945	24.11993320098619
80-81	22.605340634713045	26.785456267976937	26.657462977948455	23.951740119361567
82-83	22.86603999833515	26.44384000787462	26.977540013373485	23.71257998041674
84-85	22.940348715777496	26.54003739647476	26.56480999381384	23.95480389393391
86-87	22.497965175717976	26.80554614562202	26.734932654076225	23.96155602458378
88-89	22.666510306124373	26.340020987149472	26.76717692458662	24.226291782139533
90-91	22.66847823379132	26.43276419372483	26.674058032286176	24.224699540197676
92-93	22.69930535035715	26.422018114494968	26.68534248117699	24.19333405397089
94-95	22.315662358209583	26.358517772803207	26.898861836477124	24.426958032510086
96-97	22.395678896522757	26.328367212617504	26.80847834392861	24.467475546931126
98-99	22.211406560950262	26.53671102789128	26.63134198780674	24.620540423351716
100	22.369266131138367	26.380879546964493	26.498977549348574	24.75087677254857
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	925.0
1	710.5
2	793.0
3	1162.0
4	937.0
5	762.0
6	1323.5
7	2600.5
8	4167.0
9	5117.5
10	4729.5
11	4045.5
12	4196.0
13	4717.5
14	5335.0
15	6009.5
16	6756.5
17	7545.5
18	8313.5
19	9112.0
20	10073.5
21	11293.5
22	12780.5
23	14804.0
24	17415.0
25	20700.5
26	25433.5
27	31255.0
28	38686.0
29	48886.0
30	61960.0
31	75890.5
32	93109.5
33	111917.0
34	129371.0
35	147881.0
36	168503.0
37	193048.5
38	210045.5
39	218115.5
40	224890.0
41	230241.0
42	240512.0
43	258249.0
44	276197.0
45	298858.5
46	321260.0
47	339388.0
48	356365.5
49	363043.0
50	358611.5
51	357624.5
52	355071.5
53	344804.5
54	332792.0
55	305549.0
56	274121.0
57	240391.5
58	206700.0
59	182969.5
60	152860.5
61	121258.0
62	96264.5
63	74793.0
64	55182.0
65	38055.0
66	26988.0
67	19904.5
68	14885.5
69	11259.0
70	8511.0
71	6441.5
72	4745.5
73	3389.0
74	2481.0
75	1715.0
76	1079.0
77	636.0
78	362.5
79	224.0
80	137.5
81	61.5
82	41.5
83	31.0
84	19.5
85	13.5
86	9.5
87	9.0
88	6.0
89	6.5
90	5.0
91	1.5
92	1.5
93	1.0
94	1.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.35241284313922067
2	5.346953482112314E-4
3	0.0023210638979169365
4	0.0012516732014944735
5	0.004642127795833873
6	0.0
7	8.5065169033605E-5
8	3.0380417512001785E-4
9	0.003050193918204979
10-11	1.1544558654560678E-4
12-13	7.291300202880429E-5
14-15	3.341845926320196E-4
16-17	1.0329341954080606E-4
18-19	0.0010147059449008595
20-21	2.1266292258401246E-4
22-23	1.4582600405760857E-4
24-25	0.0
26-27	0.0021570096433521264
28-29	0.01158709123907748
30-31	0.003736791353976219
32-33	0.013179025116706374
34-35	0.010481244041640615
36-37	0.01224938434083912
38-39	0.012091406169776709
40-41	0.013543590126850394
42-43	0.006367735510515574
44-45	0.0013002818695136763
46-47	0.0057965836612899405
48-49	0.01243166684591113
50-51	0.003499824097382606
52-53	0.005304420897595512
54-55	0.004952008054456291
56-57	1.8835858857441107E-4
58-59	5.286192647088311E-4
60-61	3.8886934415362283E-4
62-63	9.964776943936586E-4
64-65	3.5848892664162103E-4
66-67	0.0012881297025088756
68-69	0.0018592815517345092
70-71	9.417929428720554E-4
72-73	0.002418281233955342
74-75	0.007680169547034051
76-77	0.0036517261849426143
78-79	0.0014704122075808862
80-81	4.617823461824271E-4
82-83	2.1266292258401246E-4
84-85	0.001634466462145696
86-87	0.004708964714360277
88-89	0.0017013033806720997
90-91	1.7013033806721E-4
92-93	0.0019504228042705146
94-95	0.002995509166683376
96-97	0.0019564988877729147
98-99	0.0021509335598497263
100	0.0027463897430849613
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	8228985.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.61565013024812
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.85418444042708	41.97541201244213
2	15.481450032034411	17.839476171092265
3	5.244891155600386	9.06563441376914
4	2.3362432702213742	5.384166995048856
5	1.1867650545650157	3.4188120085311384
6	0.713882210075856	2.4678472589963283
7	0.4739673972990011	1.911555781514649
8	0.33180689315700557	1.5293815893550922
9	0.2357973809359924	1.2227057461473279
>10	1.0796545646954745	10.62218201578652
>50	0.04455148135691209	1.738242157409062
>100	0.0157795837591623	1.4960814019871143
>500	6.580542510764462E-4	0.26178561479615564
>1k	2.8952127398830947E-4	0.2976792495411119
>5k	0.0	0.0
>10k+	7.896034732441148E-5	0.7690375835830849
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	20862	0.2535185080541525	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	19156	0.23278691114396247	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10695	0.12996742611634363	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.2152167004800713E-5
2	0.0	0.0	0.0	0.0	1.2152167004800713E-5
3	0.0	0.0	0.0	0.0	1.2152167004800713E-5
4	0.0	0.0	0.0	2.4304334009601427E-5	1.2152167004800713E-5
5	0.0	0.0	0.0	2.4304334009601427E-5	1.2152167004800713E-5
6	0.0	0.0	0.0	2.4304334009601427E-5	1.2152167004800713E-5
7	0.0	0.0	0.0	2.4304334009601427E-5	1.2152167004800713E-5
8	0.0	0.0	0.0	2.4304334009601427E-5	1.2152167004800713E-5
9	0.0	0.0	0.0	2.4304334009601427E-5	3.6456501014402144E-5
10-11	0.0	0.0	0.0	3.6456501014402144E-5	3.6456501014402144E-5
12-13	0.0	0.0	0.0	4.8608668019202854E-5	4.25325845168025E-5
14-15	6.076083502400357E-6	0.0	0.0	6.683691852640393E-5	4.8608668019202854E-5
16-17	1.2152167004800713E-5	0.0	0.0	9.114125253600536E-5	6.076083502400357E-5
18-19	1.2152167004800713E-5	0.0	0.0	1.0329341954080606E-4	9.721733603840571E-5
20-21	1.2152167004800713E-5	0.0	0.0	1.6405425456480962E-4	1.0329341954080606E-4
22-23	1.2152167004800713E-5	0.0	0.0	3.2810850912961924E-4	1.7620642156961034E-4
24-25	1.2152167004800713E-5	0.0	0.0	6.805213522688399E-4	2.3089117309121356E-4
26-27	1.2152167004800713E-5	0.0	0.0	0.0013367383705280783	2.369672565936139E-4
28-29	1.2152167004800713E-5	0.0	0.0	0.004137812865134643	2.4304334009601428E-4
30-31	1.2152167004800713E-5	0.0	0.0	0.015876806191772133	2.55195507100815E-4
32-33	1.2152167004800713E-5	1.2152167004800713E-5	0.0	0.04347437745967455	2.794998411104164E-4
34-35	1.2152167004800713E-5	2.4304334009601427E-5	0.0	0.08916044931422284	3.0380417512001785E-4
36-37	1.2152167004800713E-5	3.6456501014402144E-5	0.0	0.16214636434505592	3.0380417512001785E-4
38-39	1.2152167004800713E-5	3.6456501014402144E-5	0.0	0.2669223482604477	3.0380417512001785E-4
40-41	1.2152167004800713E-5	3.6456501014402144E-5	0.0	0.37871013253760943	3.0380417512001785E-4
42-43	1.2152167004800713E-5	3.6456501014402144E-5	0.0	0.48833483108791664	3.524128431392207E-4
44-45	1.2152167004800713E-5	3.6456501014402144E-5	0.0	0.6250467098919248	4.070975946608239E-4
46-47	1.2152167004800713E-5	4.8608668019202854E-5	0.0	0.7852122710151981	4.131736781632243E-4
48-49	1.2152167004800713E-5	4.8608668019202854E-5	0.0	0.9541942293004544	4.496301791776264E-4
50-51	1.2152167004800713E-5	4.8608668019202854E-5	0.0	1.1272653917828261	4.496301791776264E-4
52-53	1.2152167004800713E-5	5.4684751521603215E-5	0.0	1.2952569484571912	4.739345131872278E-4
54-55	1.2152167004800713E-5	7.898908553120464E-5	0.0	1.4858576118439881	5.043149306992296E-4
56-57	1.2152167004800713E-5	8.5065169033605E-5	0.0	1.706297921311073	5.286192647088311E-4
58-59	1.2152167004800713E-5	8.5065169033605E-5	0.0	1.9464551217434471	5.468475152160321E-4
60-61	1.2152167004800713E-5	8.5065169033605E-5	0.0	2.194499078561937	5.468475152160321E-4
62-63	1.2152167004800713E-5	8.5065169033605E-5	0.0	2.440592612576156	5.468475152160321E-4
64-65	1.2152167004800713E-5	8.5065169033605E-5	0.0	2.696571934448781	5.468475152160321E-4
66-67	1.2152167004800713E-5	9.721733603840571E-5	0.0	2.984110434032873	5.529235987184324E-4
68-69	1.2152167004800713E-5	9.721733603840571E-5	0.0	3.30259442689469	5.589996822208328E-4
70-71	1.2152167004800713E-5	9.721733603840571E-5	0.0	3.643651069967924	5.833040162304343E-4
72-73	1.2152167004800713E-5	9.721733603840571E-5	0.0	3.9803572372534397	6.926735192736407E-4
74-75	1.2152167004800713E-5	9.721733603840571E-5	0.0	4.320101446290156	6.926735192736407E-4
76-77	1.2152167004800713E-5	1.0936950304320642E-4	0.0	4.689733910075179	7.048256862784414E-4
78-79	1.2152167004800713E-5	1.0936950304320642E-4	0.0	5.098028493185977	7.169778532832421E-4
80-81	1.2152167004800713E-5	1.0936950304320642E-4	0.0	5.529819291200555	7.534343542976443E-4
82-83	1.2152167004800713E-5	1.0936950304320642E-4	0.0	5.966355510430509	8.628038573408506E-4
84-85	1.2152167004800713E-5	1.0936950304320642E-4	0.0	6.398766068962333	8.628038573408506E-4
86-87	1.2152167004800713E-5	1.0936950304320642E-4	0.0	6.853390788778932	8.628038573408506E-4
88	1.2152167004800713E-5	1.0936950304320642E-4	0.0	7.213064551703521	8.628038573408506E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	12795	0.0	52.306053	1
GTATCAA	21415	0.0	37.50649	1
CAACGCA	25900	0.0	30.030611	5
ATCAACG	26155	0.0	29.720398	3
GTGGTAT	5140	0.0	29.454506	1
TATCAAC	27625	0.0	28.784676	2
TCAACGC	27805	0.0	28.142319	4
AACGCAG	28225	0.0	27.972292	6
GTACTAG	3705	0.0	26.096092	1
GTACATG	28435	0.0	25.692596	1
TGGTATC	5840	0.0	24.946642	2
TACATGG	28730	0.0	24.749508	2
ACGCAGA	31780	0.0	24.459051	7
ACATGGG	28625	0.0	23.970726	3
CGCAGAG	32750	0.0	23.591116	8
CATGGGG	22065	0.0	21.512257	4
TAGTACT	5310	0.0	21.241503	4
CTGTGCG	5370	0.0	21.091175	9
CTAGTAC	5180	0.0	20.958302	3
GCAGAGT	36245	0.0	20.901379	9
>>END_MODULE