FastQCFastQC Report
Thu 26 May 2016
ERR580072_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580072_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6193118
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT122540.1978647912085641No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT99180.16014550344430706No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA92900.049.7515261
GTATCAA166700.041.2081571
CAACGCA194000.034.0231325
ATCAACG199100.033.1298833
TATCAAC206650.032.695652
AACGCAG211950.031.7277816
TCAACGC211400.031.4014114
ACGCAGA240300.027.0846047
CGCAGAG247200.026.1385028
GTGGTAT41050.026.1150131
GTACATG260350.023.3873351
GCAGAGT274350.022.921389
TACATGG262050.022.715282
GTACTAG30250.022.2269171
ACATGGG265800.021.6324123
GAGTACT116650.020.996812-13
TGGTATC48250.020.4642
CAGAGTA266900.019.6709710-11
TGATCGC20600.019.3928369
CATGGGG205450.019.26754
Produced by FastQC (version 0.11.5)