FastQCFastQC Report
Thu 26 May 2016
ERR580072_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580072_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6193118
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT111770.18047452026588223No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT95630.15441333428492723No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA94500.049.8960651
GTATCAA168200.039.9752921
CAACGCA197700.033.184355
ATCAACG201000.032.6166763
TATCAAC211250.031.611742
TCAACGC213600.030.8241714
AACGCAG217250.030.6084046
ACGCAGA242600.026.9645087
CGCAGAG251650.025.8267218
GTGGTAT42950.023.6033361
GTACTAG30500.023.4985921
GTACATG262550.023.2571141
GCAGAGT278000.023.0579459
TACATGG266800.022.4582232
ACATGGG265950.021.6646673
GAGTACT118100.021.30870812-13
GTATAAG33100.020.3707081
TGGTATC46550.020.1911642
CATGGGG198050.019.8374564
AGAGTAC252850.019.4034110-11
Produced by FastQC (version 0.11.5)