##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR580072_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6193118
Sequences flagged as poor quality	0
Sequence length	100
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.53304022303467	34.0	31.0	34.0	31.0	34.0
2	32.73598371611844	34.0	31.0	34.0	31.0	34.0
3	32.815522003617566	34.0	31.0	34.0	31.0	34.0
4	36.28634768463963	37.0	37.0	37.0	35.0	37.0
5	36.2170402695379	37.0	37.0	37.0	35.0	37.0
6	36.24338241254244	37.0	37.0	37.0	35.0	37.0
7	36.212755513458646	37.0	37.0	37.0	35.0	37.0
8	36.20200487056762	37.0	37.0	37.0	35.0	37.0
9	37.986842007531585	39.0	38.0	39.0	35.0	39.0
10-11	37.93791665199985	39.0	38.0	39.0	35.0	39.0
12-13	37.90280606634655	39.0	38.0	39.0	35.0	39.0
14-15	39.30703928134423	41.0	39.0	41.0	36.0	41.0
16-17	39.29463091127926	41.0	39.0	41.0	36.0	41.0
18-19	39.2939987579762	41.0	39.0	41.0	36.0	41.0
20-21	39.27527200353683	41.0	39.0	41.0	36.0	41.0
22-23	39.20875114925955	41.0	39.0	41.0	36.0	41.0
24-25	39.13026951852039	40.5	39.0	41.0	35.5	41.0
26-27	38.99584127090748	40.0	38.5	41.0	35.0	41.0
28-29	38.89360512749798	40.0	38.0	41.0	35.0	41.0
30-31	38.787960119603724	40.0	38.0	41.0	35.0	41.0
32-33	38.65327158307011	40.0	38.0	41.0	35.0	41.0
34-35	38.62956099011839	40.0	38.0	41.0	34.5	41.0
36-37	38.68614307042107	40.0	38.0	41.0	35.0	41.0
38-39	38.60093340704957	40.0	38.0	41.0	35.0	41.0
40-41	38.48145029692637	40.0	38.0	41.0	34.0	41.0
42-43	38.45990937036885	40.0	38.0	41.0	34.0	41.0
44-45	38.37903540672082	40.0	38.0	41.0	34.0	41.0
46-47	38.192420118589695	40.0	38.0	41.0	33.5	41.0
48-49	37.99138083595372	40.0	37.0	41.0	33.0	41.0
50-51	37.82445490300685	40.0	37.0	41.0	33.0	41.0
52-53	37.649901794217385	40.0	36.5	41.0	33.0	41.0
54-55	37.454331727572445	40.0	36.0	41.0	33.0	41.0
56-57	37.243607420365635	39.0	35.5	41.0	32.0	41.0
58-59	37.0155038221458	39.0	35.0	41.0	32.0	41.0
60-61	36.75784992309205	39.0	35.0	40.5	31.5	41.0
62-63	36.471282639859275	38.0	35.0	40.0	31.0	41.0
64-65	36.17997993902264	37.5	35.0	40.0	31.0	41.0
66-67	35.84918154312577	37.0	35.0	40.0	31.0	41.0
68-69	35.4983407388653	36.0	35.0	39.0	30.5	41.0
70-71	35.16662455002472	36.0	34.0	39.0	30.0	40.5
72-73	34.81282546206935	35.0	34.0	38.5	30.0	40.0
74-75	34.45519478233743	35.0	34.0	37.0	29.0	39.5
76-77	33.22386235818533	34.5	32.5	36.0	28.0	39.0
78-79	33.84803938823707	35.0	34.0	36.0	29.0	39.0
80-81	33.77979767219033	35.0	34.0	36.0	29.0	37.5
82-83	33.57264079257008	35.0	34.0	36.0	29.0	37.0
84-85	33.35455137460646	35.0	34.0	35.0	29.0	37.0
86-87	33.186471015084805	35.0	34.0	35.0	29.0	36.0
88-89	33.027819347217346	35.0	33.5	35.0	29.0	36.0
90-91	32.87233417803439	35.0	33.0	35.0	29.0	36.0
92-93	32.760988971952415	35.0	33.0	35.0	29.0	35.5
94-95	32.66464622505174	35.0	33.0	35.0	29.0	35.0
96-97	32.57346331847705	35.0	33.0	35.0	27.5	35.0
98-99	32.5020079546361	35.0	33.0	35.0	27.0	35.0
100	31.23088805994008	34.0	31.0	35.0	24.0	35.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.1152609071556725
1101	2	0.07485906580511426
1101	3	0.05781263714818152
1101	4	0.04019960679583079
1101	5	-0.005554521580975802
1101	6	0.017674228963436178
1101	7	0.016628915716033532
1101	8	0.002439432905710248
1101	9	0.04262612181606329
1101	10-11	-0.00771689653303298
1101	12-13	0.0073261152528019124
1101	14-15	0.10176347422757459
1101	16-17	0.025168087342102297
1101	18-19	0.005474321897274592
1101	20-21	-0.03923775538212482
1101	22-23	-0.04478008539683742
1101	24-25	-0.04734411261676996
1101	26-27	-0.0717440675099752
1101	28-29	-0.01573277833917075
1101	30-31	-0.05524291573847506
1101	32-33	-0.08317061174005147
1101	34-35	-0.08000440968599065
1101	36-37	0.04751647636611267
1101	38-39	0.01694234823974483
1101	40-41	0.06145796345926158
1101	42-43	0.02995659559080366
1101	44-45	0.010254673107901624
1101	46-47	0.03523156125320526
1101	48-49	0.06665494999450772
1101	50-51	0.052242113879806595
1101	52-53	-0.03752680716349488
1101	54-55	-0.04345515444293113
1101	56-57	-0.04328243020726319
1101	58-59	-0.05474440103428435
1101	60-61	-0.13358568417001493
1101	62-63	-0.07847135320649556
1101	64-65	-0.02143706606712925
1101	66-67	-0.06272813713719216
1101	68-69	1.3635892635477376E-5
1101	70-71	-0.07362830952865096
1101	72-73	-0.0542260944887758
1101	74-75	-0.009434419919884363
1101	76-77	0.0100110468218233
1101	78-79	-0.008335683688024176
1101	80-81	0.038345726514357636
1101	82-83	0.04650437738313684
1101	84-85	0.027209251726887373
1101	86-87	0.007465420683104185
1101	88-89	-0.06093134593614735
1101	90-91	0.003968121994425644
1101	92-93	-0.08535382703239947
1101	94-95	-0.014610428056727187
1101	96-97	0.029294242072523957
1101	98-99	-0.020945816701313902
1101	100	-0.0038322786737445824
1102	1	0.05167311200472113
1102	2	-0.022151146383009745
1102	3	-0.028613256293837708
1102	4	0.001276814442327634
1102	5	-0.03822575841255116
1102	6	-0.0037333366970173643
1102	7	-0.0010666519673421249
1102	8	-0.01517196237505658
1102	9	0.005312460898942106
1102	10-11	-0.01192412291548095
1102	12-13	0.002425972294112455
1102	14-15	0.0043286418854719955
1102	16-17	-0.060022622135186054
1102	18-19	-0.018457494972246025
1102	20-21	-0.06594579312481841
1102	22-23	-0.07385647371882698
1102	24-25	-0.06523375966013134
1102	26-27	-0.07368936590139441
1102	28-29	-0.11027593274848613
1102	30-31	-0.08911182979004195
1102	32-33	-0.0896827482954663
1102	34-35	-0.08231248067951924
1102	36-37	-0.023904976994117533
1102	38-39	-0.06856304954578718
1102	40-41	-0.050600193647952096
1102	42-43	-0.01792391245012226
1102	44-45	-0.07167835034886849
1102	46-47	-0.0863597302132817
1102	48-49	-0.1165417297003799
1102	50-51	-0.12969173089253871
1102	52-53	-0.12843121001231594
1102	54-55	-0.1616132970153501
1102	56-57	-0.1734228701934839
1102	58-59	-0.22449470118780113
1102	60-61	-0.18109607754533386
1102	62-63	-0.2662861410681501
1102	64-65	-0.24848385915334603
1102	66-67	-0.1747991864101479
1102	68-69	-0.09937406089956369
1102	70-71	-0.16717724898624198
1102	72-73	-0.22736877641764153
1102	74-75	-0.1971062490942117
1102	76-77	-0.19489198318556333
1102	78-79	-0.1369843408121696
1102	80-81	-0.12773931598498933
1102	82-83	-0.12593341667272995
1102	84-85	-0.0963103891640742
1102	86-87	-0.1155839797067415
1102	88-89	-0.0757585560714844
1102	90-91	-0.11592663014747018
1102	92-93	-0.0978612188726089
1102	94-95	-0.17821029405784827
1102	96-97	-0.1867467231574551
1102	98-99	-0.1536824601189437
1102	100	-0.1284249061469005
1103	1	0.08604438331935427
1103	2	0.08857302640574005
1103	3	0.05308808426617162
1103	4	0.03416984924526645
1103	5	-0.007860727476945328
1103	6	0.04378111203877921
1103	7	0.028972251823489614
1103	8	0.02752613867750142
1103	9	0.008145903308324876
1103	10-11	-0.0231560882405617
1103	12-13	-0.040765774694520474
1103	14-15	0.04073747171965891
1103	16-17	0.0427187585525175
1103	18-19	0.03102650618303926
1103	20-21	0.02104355941409608
1103	22-23	-0.035504041835025646
1103	24-25	-0.0024170166402370796
1103	26-27	-0.04657345698040416
1103	28-29	-0.03556381926433261
1103	30-31	-0.04934549321924919
1103	32-33	-0.08420751470372778
1103	34-35	-0.14290016758106816
1103	36-37	-0.059186034734779014
1103	38-39	-0.08720994579449126
1103	40-41	-0.06838581158682189
1103	42-43	-0.0644805403307771
1103	44-45	-0.03979840415971836
1103	46-47	-0.008895379645927903
1103	48-49	-0.03224924406718088
1103	50-51	-0.05345090121812035
1103	52-53	-0.08501653564785272
1103	54-55	-0.026043955885896253
1103	56-57	-0.019809354800450762
1103	58-59	-0.011708171721387828
1103	60-61	-0.004191868336441473
1103	62-63	-0.022743225531932865
1103	64-65	-0.048324789915149324
1103	66-67	-0.002932306302653842
1103	68-69	-0.07258536732337717
1103	70-71	-0.05948661938699473
1103	72-73	-0.07235486282021242
1103	74-75	-0.13436820213927803
1103	76-77	-0.12621799715754634
1103	78-79	-0.05274650842466855
1103	80-81	-0.009030981243398628
1103	82-83	0.023248999448817642
1103	84-85	0.00617897720137961
1103	86-87	0.0799063657826764
1103	88-89	0.06867412301039622
1103	90-91	-0.0019270009036134184
1103	92-93	-0.009855026630425812
1103	94-95	-0.04211550594703084
1103	96-97	-0.03898676369482956
1103	98-99	-0.08798329288641327
1103	100	0.010846542659560754
1104	1	0.09323571777411388
1104	2	0.049039595912375944
1104	3	0.040774331862238
1104	4	0.035099707771557576
1104	5	-0.003539122142704798
1104	6	0.042360187224893764
1104	7	0.019115018317428678
1104	8	-0.0011010407663505362
1104	9	0.015857216117240114
1104	10-11	-0.0268764774764918
1104	12-13	-0.039906647473912926
1104	14-15	-0.007338751639579755
1104	16-17	0.008540552679967561
1104	18-19	-0.01027053455594995
1104	20-21	-0.070303185555737
1104	22-23	-0.04277314200452054
1104	24-25	-0.07173949153068548
1104	26-27	-0.06656950686930685
1104	28-29	-0.09747392436976554
1104	30-31	-0.05117807939508623
1104	32-33	-0.04780018849964307
1104	34-35	-0.1365327883016576
1104	36-37	-0.08402450727829347
1104	38-39	-0.07623661616913324
1104	40-41	-0.037345287737345245
1104	42-43	-0.04689422083917805
1104	44-45	-0.08401006516542964
1104	46-47	-0.16018472487048285
1104	48-49	-0.10555935120161308
1104	50-51	-0.06063549812879643
1104	52-53	-0.09363419011462781
1104	54-55	-0.17339085687763145
1104	56-57	-0.03020343633520639
1104	58-59	0.0032009549429190542
1104	60-61	-0.039435754912240384
1104	62-63	-0.05043626126819589
1104	64-65	-0.11323268910734186
1104	66-67	-0.1395506621968181
1104	68-69	-0.023013770578863557
1104	70-71	-0.07699060402477187
1104	72-73	-0.09545246755616432
1104	74-75	-0.17205930154151616
1104	76-77	-0.15921338973577548
1104	78-79	-0.08829845048818896
1104	80-81	-0.0810654983270851
1104	82-83	0.023656517608365846
1104	84-85	0.07005305059223588
1104	86-87	0.0167899176073405
1104	88-89	-0.014403370801836957
1104	90-91	-0.0060488548351642635
1104	92-93	-0.008408300378569322
1104	94-95	-0.03493269022616374
1104	96-97	-0.04723829330448126
1104	98-99	-0.09090434092507849
1104	100	-0.07895880194761773
1105	1	0.1161270955737379
1105	2	0.0574345603989741
1105	3	0.027527452451501233
1105	4	0.013298661219110386
1105	5	-0.011609306014960907
1105	6	0.01232995980005569
1105	7	-0.009657346178762793
1105	8	-0.02612135883489941
1105	9	-0.03459424153307111
1105	10-11	-0.012323933209579252
1105	12-13	-0.03339765476039247
1105	14-15	0.022640807541556285
1105	16-17	-0.019703934063706185
1105	18-19	-0.04526205964308616
1105	20-21	-0.08436830183241995
1105	22-23	-0.01831044453879116
1105	24-25	-0.03138502462114445
1105	26-27	-0.06560372541447634
1105	28-29	-0.08087349359971796
1105	30-31	-0.16172010749500032
1105	32-33	-0.07834182842581328
1105	34-35	-0.08318483461361836
1105	36-37	-0.10020589033625527
1105	38-39	-0.0706178781220217
1105	40-41	-0.10069746892040143
1105	42-43	-0.11216389041976527
1105	44-45	-0.12455829761878334
1105	46-47	-0.11328152242411704
1105	48-49	-0.1430717435203519
1105	50-51	-0.10399652504633394
1105	52-53	-0.05519529644069365
1105	54-55	-0.08140696634571754
1105	56-57	-0.1040105250307235
1105	58-59	-0.0762142934220904
1105	60-61	-0.10708840006576992
1105	62-63	-0.06256705114653016
1105	64-65	-0.0792150045792468
1105	66-67	-0.12280242976917322
1105	68-69	-0.03943375196451626
1105	70-71	-0.05935596010800026
1105	72-73	-0.14436045893562977
1105	74-75	-0.1768858052231863
1105	76-77	-0.127366689064921
1105	78-79	-0.07287107567776019
1105	80-81	-0.0617976454511151
1105	82-83	-0.0795532552187197
1105	84-85	-0.05802179650650885
1105	86-87	-0.019836808721549914
1105	88-89	-0.04166586790821469
1105	90-91	-0.03292060749495107
1105	92-93	-0.11241222273302043
1105	94-95	-0.12302366067471127
1105	96-97	-0.14323605475011192
1105	98-99	-0.15802453250296367
1105	100	-0.01897467079938764
1106	1	0.10606078643844796
1106	2	0.07184017758714845
1106	3	0.014273550036563165
1106	4	0.05336301435126245
1106	5	0.002291901360990778
1106	6	0.04823928427455826
1106	7	0.036976913310525106
1106	8	0.05315802696753025
1106	9	0.015450381753936426
1106	10-11	0.03663262402363898
1106	12-13	0.046780486181262404
1106	14-15	0.05352376999950792
1106	16-17	0.006020840571402175
1106	18-19	2.9916278570851773E-5
1106	20-21	0.0215488906804282
1106	22-23	6.347351713529292E-4
1106	24-25	0.0029055971928784174
1106	26-27	0.01752571608059128
1106	28-29	-0.005731211667338698
1106	30-31	-0.020007177056385217
1106	32-33	-0.011229348182382637
1106	34-35	0.01497432114775421
1106	36-37	0.032597917517563246
1106	38-39	-0.044656622078697694
1106	40-41	-0.05849880818506392
1106	42-43	-0.01856385721173126
1106	44-45	-0.047820206438849766
1106	46-47	-0.0073977266207805314
1106	48-49	0.010661926840917602
1106	50-51	-0.005562711223873862
1106	52-53	-0.04321273317091112
1106	54-55	-0.03486220500639803
1106	56-57	-0.014981878704396934
1106	58-59	-0.01711482217188376
1106	60-61	-0.011811487255080522
1106	62-63	-0.04854037025743452
1106	64-65	0.04418973649563185
1106	66-67	-0.030668015246192226
1106	68-69	-0.034107825866207975
1106	70-71	-0.035931533213776845
1106	72-73	-0.10678922362844645
1106	74-75	-0.0626253473675078
1106	76-77	-0.05952721717319065
1106	78-79	-0.023699295205467763
1106	80-81	-0.001798286969822982
1106	82-83	0.02112191178054701
1106	84-85	0.059128766048800685
1106	86-87	0.051670011817364525
1106	88-89	0.07370951173564322
1106	90-91	0.09247120634889683
1106	92-93	0.037540135068375946
1106	94-95	0.02577603894359015
1106	96-97	0.03983143616327567
1106	98-99	-0.005383142092917126
1106	100	-0.01146349296474014
1107	1	0.06315131827732046
1107	2	0.03406219663306587
1107	3	-0.019681938180561076
1107	4	-0.006969651610091887
1107	5	-0.01244899597754312
1107	6	-0.01740163228279812
1107	7	-0.003339195854856314
1107	8	-0.05010799073741623
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2316	7	0.025113931596301597
2316	8	0.037120901153421926
2316	9	0.017942260969192603
2316	10-11	-0.0016457787805777002
2316	12-13	0.032085183801086714
2316	14-15	0.06938621670810363
2316	16-17	0.027961125325496994
2316	18-19	0.03325973649238989
2316	20-21	0.04308491840352957
2316	22-23	0.018408887156503795
2316	24-25	0.03060520368638464
2316	26-27	0.01714589418198642
2316	28-29	0.025055404756777477
2316	30-31	-0.27868075509098844
2316	32-33	-0.08043476627185697
2316	34-35	-0.1993908662612327
2316	36-37	-0.4925675181853677
2316	38-39	-0.3088823619004657
2316	40-41	-0.2587760414954232
2316	42-43	-0.33550925477661053
2316	44-45	-0.15754668424288099
2316	46-47	-0.3362902649886408
2316	48-49	-0.29161454040740864
2316	50-51	-0.27139753660551236
2316	52-53	-0.3346722074121331
2316	54-55	-0.14977670152691758
2316	56-57	-0.941685719169115
2316	58-59	-0.6634183665726496
2316	60-61	-0.3787059859348787
2316	62-63	-0.1377483166725213
2316	64-65	-0.040850002685694164
2316	66-67	-0.04772066607984016
2316	68-69	-0.17973101457764074
2316	70-71	-0.1395934678848718
2316	72-73	-0.049639838552543836
2316	74-75	-0.051517894904272055
2316	76-77	-0.04455722585385047
2316	78-79	-0.14182128107114522
2316	80-81	-0.11606934410563241
2316	82-83	-0.32830624652334706
2316	84-85	-0.21127115409004205
2316	86-87	-0.24850399108138532
2316	88-89	-0.2611838458738944
2316	90-91	-0.157947468785558
2316	92-93	-0.26116954782273893
2316	94-95	-0.13146454235583604
2316	96-97	-0.408890819589125
2316	98-99	-0.14129460347658096
2316	100	-0.32986555450138866
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	3.0
12	6.0
13	57.0
14	184.0
15	590.0
16	1444.0
17	2922.0
18	5505.0
19	8646.0
20	13294.0
21	20806.0
22	27857.0
23	30676.0
24	31065.0
25	32581.0
26	37367.0
27	46322.0
28	57398.0
29	71564.0
30	88121.0
31	110185.0
32	140624.0
33	185580.0
34	272312.0
35	418958.0
36	751413.0
37	1574895.0
38	1887487.0
39	375198.0
40	58.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.550063359033444	19.844665039749287	14.164940902712287	23.440330698504987
2	15.676436855198803	20.454713163426792	39.42199460657045	24.446855374803956
3	18.761444365287026	25.64059380552636	30.18257375580575	25.415388073380868
4	13.276251940084883	17.379382105167295	38.47184285296304	30.872523101784783
5	13.976500299942918	37.66157557633043	34.0888557682293	14.273068355497351
6	30.529419914169242	36.89572845213025	18.27468812963034	14.300163504070163
7	27.664808149701848	32.461186705871526	21.889616041052072	17.984389103374557
8	26.74241982646162	34.21735522631084	19.967457315782394	19.07276763144514
9	26.374175388193667	16.23757410485597	20.573174437752566	36.815076069197794
10-11	25.31449251083361	25.920302594212526	27.6962432056579	21.068961689295975
12-13	26.064179824222528	23.380917630378057	27.787763329743054	22.767139215656353
14-15	23.604621591269645	24.9006516117521	25.36671831012069	26.128008486857563
16-17	22.943865889782444	27.761942092145876	26.10064563632356	23.193546381748117
18-19	22.597214976199943	27.18399955563112	27.966586108660785	22.252199359508147
20-21	23.794781595225462	26.233140610771716	27.72392079281142	22.24815700119141
22-23	23.74954011404445	25.9595600692802	27.41329620821456	22.877603608460788
24-25	23.361552290784708	26.202568722249435	27.623452354694354	22.8124266322715
26-27	23.168200619135657	26.473430396326425	27.29854899617527	23.059819988362644
28-29	22.895455100751715	26.49325373393673	27.625863242117326	22.985427923194234
30-31	23.478102412841316	26.190222635312733	27.510132393050057	22.821542558795898
32-33	23.423477181399786	26.403451668868072	27.154656683005907	23.018414466726238
34-35	23.502846582030486	26.468097764260907	27.385952824793318	22.64310282891529
36-37	23.46296003969425	26.262300998856247	27.23416418611242	23.04057477533708
38-39	23.453546669000733	26.335390095357354	27.34562233637583	22.865440899266083
40-41	23.49104593657437	25.914932784065613	27.596051803187034	22.997969476172976
42-43	23.31495528454891	25.93801360760403	27.39405533664238	23.352975771204676
44-45	23.328063968687378	26.31826424222069	27.26168773605166	23.091984053040274
46-47	23.319655506731422	26.18721930877859	26.895378271487875	23.597746913002112
48-49	23.603007209415406	26.07196916050352	27.070900255623137	23.25412337445794
50-51	23.504140355897103	26.254449561517003	26.838167765252564	23.403242317333333
52-53	23.28803920651995	26.37172801627323	26.79553842475845	23.54469435244836
54-55	23.21997292009976	26.26677657951955	26.893182954034412	23.62006754634627
56-57	23.57522882087404	26.2276286617617	26.645181494882582	23.55196102248168
58-59	23.137542224547396	26.354116207121496	27.058547604745943	23.449793963585165
60-61	23.28984433236449	26.52611612749256	26.778259703119527	23.40577983702342
62-63	23.367067330205103	26.252200043274243	26.664992749949135	23.71573987657152
64-65	22.992946264484765	26.153575877934504	26.934581969954795	23.918895887625933
66-67	23.226423795548023	26.167813979083565	26.788794940888327	23.816967284480086
68-69	23.022695092033555	26.219660769321813	26.86470220553388	23.892941933110748
70-71	23.04862402823677	25.963080122191624	26.866575397830427	24.12172045174118
72-73	23.116130228208103	26.204370651502746	26.746188888515704	23.93331023177345
74-75	23.124058050492128	26.331995125156453	26.684552504445218	23.859394319906198
76-77	22.925159291979668	26.284286792252963	26.551424516536994	24.239129399230375
78-79	22.891311313950794	26.216639120841574	26.68694327890614	24.205106286301493
80-81	22.823254646486447	26.485140261486446	26.659205129068376	24.032399962958735
82-83	23.13318662183618	26.138834675389916	26.972487315332998	23.75549138744091
84-85	23.162798808821695	26.181621186083863	26.585220233561046	24.07035977153339
86-87	22.600392727635608	26.451799226574746	26.84865259348233	24.099155452307315
88-89	22.827191373895317	25.91020481056442	26.87307984292951	24.389523972610757
90-91	22.86259477273516	25.982096218294377	26.863318157854582	24.291990851115877
92-93	22.85186245525378	26.09246661664229	26.826022879773088	24.229648048330844
94-95	22.450920092344294	25.939481449031	27.018715431240654	24.590883027384052
96-97	22.452926935072153	25.920072249249575	26.999951800462636	24.627049015215633
98-99	22.255698284308085	26.04376001385439	26.91328451194558	24.787257189891946
100	22.48157385032856	25.812902220503425	26.740309367160965	24.965214562007052
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	197.0
1	144.5
2	155.5
3	243.0
4	197.5
5	164.5
6	354.0
7	703.0
8	1208.0
9	1597.5
10	1531.0
11	1396.5
12	1481.5
13	1729.0
14	2064.0
15	2438.5
16	2855.5
17	3355.5
18	3795.5
19	4121.0
20	4569.5
21	5115.0
22	5765.5
23	6699.5
24	7939.5
25	9418.5
26	11577.5
27	14367.5
28	18168.0
29	23950.5
30	31738.0
31	41178.0
32	54349.5
33	70525.0
34	87772.0
35	106255.0
36	129848.0
37	158530.0
38	178653.0
39	188576.5
40	192159.5
41	193286.5
42	201488.5
43	215105.0
44	228256.0
45	246619.5
46	263529.0
47	274982.0
48	284629.5
49	285700.0
50	278326.0
51	272572.5
52	265834.0
53	256304.5
54	246885.0
55	225850.5
56	200270.5
57	172611.5
58	146835.5
59	128320.0
60	105991.0
61	83802.0
62	65989.0
63	50416.0
64	35938.5
65	24050.5
66	16942.0
67	12278.0
68	9205.5
69	6926.5
70	5120.0
71	3883.0
72	2787.0
73	1922.5
74	1357.0
75	934.5
76	612.0
77	324.0
78	186.5
79	99.5
80	58.0
81	37.0
82	20.5
83	12.0
84	8.0
85	5.0
86	3.5
87	3.0
88	2.5
89	3.0
90	2.5
91	1.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.35423190709429403
2	4.3596779522043664E-4
3	0.0018407529131529545
4	0.0014532259840681222
5	0.00440811881833997
6	0.0
7	1.2917564302827753E-4
8	3.3908606294922846E-4
9	0.003293978897221077
10-11	8.88082545819408E-5
12-13	5.651434382487141E-5
14-15	2.0991041992095099E-4
16-17	4.844086613560407E-5
18-19	0.0010495520996047548
20-21	2.9871867450289175E-4
22-23	1.0495520996047549E-4
24-25	0.0
26-27	0.0020910307215202422
28-29	0.011181766599635273
30-31	0.0037541671255093153
32-33	0.013999410313189577
34-35	0.011165619644256738
36-37	0.012683433449838999
38-39	0.01258655171756779
40-41	0.014104365523150052
42-43	0.006523369972928015
44-45	0.0014209320733110527
46-47	0.005974373490057835
48-49	0.012901417347449217
50-51	0.0037541671255093153
52-53	0.0060147408785041715
54-55	0.005376936141052052
56-57	1.5339607609607953E-4
58-59	6.135843043843181E-4
60-61	3.148656298814264E-4
62-63	9.36523411955012E-4
64-65	2.6642476374582236E-4
66-67	0.0015178138055822609
68-69	0.0016954303147461425
70-71	9.445968896442793E-4
72-73	0.002809570235865036
74-75	0.008218800287674157
76-77	0.0039560040677409985
78-79	0.0016873568370568753
80-81	3.9560040677409994E-4
82-83	1.776165091638816E-4
84-85	0.0014612994617573894
86-87	0.004464633162164841
88-89	0.0016550629262998059
90-91	2.0991041992095099E-4
92-93	0.0022121328868592526
94-95	0.003342419763356681
96-97	0.0018165324800851524
98-99	0.0021879124537914504
100	0.0024704841729158073
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	6193118.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.27380732275365
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.80919121861146	45.090347774816216
2	14.693907571380926	17.7131550755153
3	4.844383537166644	8.759683197501063
4	2.084110389743642	5.024690722830291
5	1.100254941083044	3.315827716237353
6	0.62078831505313	2.245036517383755
7	0.395648476877868	1.6693068044014296
8	0.2711427303598209	1.3074243749338552
9	0.20214977618317292	1.0965902994002106
>10	0.9188413465448627	9.341629053181787
>50	0.04214694233114963	1.7316813725844642
>100	0.016361266213486252	1.7473162815061993
>500	8.457798903577195E-4	0.33090811312128526
>1k	1.6264897163316606E-4	0.21975464577920148
>5k	3.2529794326514726E-5	0.18750122034104524
>10k+	3.2529794326514726E-5	0.21914683046657563
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11177	0.18047452026588223	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9563	0.15441333428492723	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.614695537853469E-5
2	0.0	0.0	0.0	0.0	3.229391075706938E-5
3	0.0	0.0	0.0	0.0	3.229391075706938E-5
4	0.0	0.0	0.0	0.0	3.229391075706938E-5
5	0.0	0.0	0.0	0.0	3.229391075706938E-5
6	0.0	0.0	0.0	0.0	3.229391075706938E-5
7	0.0	0.0	0.0	1.614695537853469E-5	4.844086613560407E-5
8	0.0	0.0	0.0	4.844086613560407E-5	4.844086613560407E-5
9	0.0	0.0	0.0	1.1302868764974283E-4	6.458782151413876E-5
10-11	0.0	0.0	0.0	1.9376346454241626E-4	6.458782151413876E-5
12-13	0.0	0.0	0.0	2.4220433067802035E-4	6.458782151413876E-5
14-15	0.0	0.0	0.0	2.6642476374582236E-4	1.1302868764974283E-4
16-17	0.0	0.0	0.0	2.825717191243571E-4	1.1302868764974283E-4
18-19	0.0	0.0	0.0	3.3101258525996115E-4	1.1302868764974283E-4
20-21	0.0	0.0	0.0	4.1174736215263455E-4	1.1302868764974283E-4
22-23	0.0	0.0	0.0	5.409230051809121E-4	1.5339607609607956E-4
24-25	0.0	0.0	0.0	0.0010172581888476854	2.0991041992095099E-4
26-27	0.0	0.0	0.0	0.0016954303147461423	2.2605737529948566E-4
28-29	1.614695537853469E-5	0.0	0.0	0.0035523301832776316	2.2605737529948566E-4
30-31	3.229391075706938E-5	0.0	0.0	0.008670915038273129	2.4220433067802035E-4
32-33	3.229391075706938E-5	0.0	0.0	0.02208096148014619	2.906451968136244E-4
34-35	3.229391075706938E-5	0.0	0.0	0.04571203067663171	3.794534513955652E-4
36-37	3.229391075706938E-5	0.0	0.0	0.08479573617037492	4.0367388446336725E-4
38-39	3.229391075706938E-5	0.0	0.0	0.14333652289525245	4.0367388446336725E-4
40-41	3.229391075706938E-5	0.0	0.0	0.2105966655245387	4.0367388446336725E-4
42-43	3.229391075706938E-5	0.0	0.0	0.2818370326546337	4.1174736215263455E-4
44-45	3.229391075706938E-5	0.0	0.0	0.37175135368000417	4.359677952204366E-4
46-47	3.229391075706938E-5	0.0	0.0	0.4807917433512489	5.570699605594468E-4
48-49	3.229391075706938E-5	0.0	0.0	0.6006586666038012	6.781721258984569E-4
50-51	3.229391075706938E-5	0.0	0.0	0.7285829205902423	6.781721258984569E-4
52-53	3.229391075706938E-5	0.0	0.0	0.8542466008236885	7.26612992034061E-4
54-55	3.229391075706938E-5	0.0	0.0	0.9965981594408504	7.831273358589325E-4
56-57	3.229391075706938E-5	0.0	0.0	1.1659393539732328	7.912008135481998E-4
58-59	3.229391075706938E-5	0.0	0.0	1.3582334455762024	7.912008135481998E-4
60-61	3.229391075706938E-5	0.0	0.0	1.5589723948421457	7.912008135481998E-4
62-63	3.229391075706938E-5	0.0	0.0	1.7649106637399772	7.912008135481998E-4
64-65	3.229391075706938E-5	0.0	0.0	1.9797136111406242	7.992742912374671E-4
66-67	3.229391075706938E-5	0.0	0.0	2.2215707822780058	8.234947243052691E-4
68-69	3.229391075706938E-5	0.0	0.0	2.493081836968067	8.477151573730711E-4
70-71	3.229391075706938E-5	0.0	0.0	2.7909931637020318	9.284499342657447E-4
72-73	3.229391075706938E-5	0.0	0.0	3.0937889444380033	0.001146433831875963
74-75	3.229391075706938E-5	0.0	0.0	3.396778488638518	0.001146433831875963
76-77	3.229391075706938E-5	0.0	0.0	3.7243436989251615	0.0011787277426330324
78-79	3.229391075706938E-5	0.0	0.0	4.090524352999571	0.0012110216533901016
80-81	3.229391075706938E-5	0.0	0.0	4.480392590614292	0.0012594625195257057
82-83	3.229391075706938E-5	0.0	0.0	4.890016628134649	0.0013886381625539833
84-85	3.229391075706938E-5	0.0	0.0	5.298784877019944	0.0013967116402432505
86-87	3.229391075706938E-5	0.0	0.0	5.7288267396164585	0.001404785117932518
88	3.229391075706938E-5	0.0	0.0	6.067622157368873	0.001404785117932518
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	9450	0.0	49.896065	1
GTATCAA	16820	0.0	39.975292	1
CAACGCA	19770	0.0	33.18435	5
ATCAACG	20100	0.0	32.616676	3
TATCAAC	21125	0.0	31.61174	2
TCAACGC	21360	0.0	30.824171	4
AACGCAG	21725	0.0	30.608404	6
ACGCAGA	24260	0.0	26.964508	7
CGCAGAG	25165	0.0	25.826721	8
GTGGTAT	4295	0.0	23.603336	1
GTACTAG	3050	0.0	23.498592	1
GTACATG	26255	0.0	23.257114	1
GCAGAGT	27800	0.0	23.057945	9
TACATGG	26680	0.0	22.458223	2
ACATGGG	26595	0.0	21.664667	3
GAGTACT	11810	0.0	21.308708	12-13
GTATAAG	3310	0.0	20.370708	1
TGGTATC	4655	0.0	20.191164	2
CATGGGG	19805	0.0	19.837456	4
AGAGTAC	25285	0.0	19.40341	10-11
>>END_MODULE