FastQCFastQC Report
Thu 26 May 2016
ERR580071_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580071_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6803340
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT96080.14122475137212015No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74520.109534434557144No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA88700.044.4730031
GTATCAA160350.042.3112141
CAACGCA183950.035.446565
ATCAACG190500.034.2063833
TATCAAC199000.033.786832
AACGCAG210650.031.67856
TCAACGC211250.031.000894
GTGGTAT39300.027.8754221
ACGCAGA232800.027.5731457
CGCAGAG240300.026.7323118
GTACTAG33700.025.9502281
GCAGAGT269700.023.2466589
GTACATG270350.023.1130221
TACATGG274150.022.2613222
ACATGGG275650.021.2690143
TGGTATC48000.020.4724372
GTATAAG40900.020.1174451
TAGGCAT51500.019.8084245
GCATCGT38000.019.5386818
CATGGGG226650.019.2699134
Produced by FastQC (version 0.11.5)