FastQCFastQC Report
Thu 26 May 2016
ERR580071_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580071_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6803340
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87000.12787836562629532No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72290.10625663277155045No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA89700.046.005661
GTATCAA156500.041.768091
CAACGCA181450.034.5006525
ATCAACG189500.032.986183
TATCAAC199700.032.3119282
AACGCAG207750.030.9691776
TCAACGC210300.029.8796394
GTACTAG34850.027.3365881
ACGCAGA231950.026.887097
CGCAGAG242950.025.5536638
GTACATG273400.022.8739261
GTGGTAT36600.022.6791761
GCAGAGT273450.022.4111299
TACATGG276900.022.1322272
ACATGGG283200.021.0267893
TGATCGC22150.020.5807939
GTATAAG39200.020.4530741
TAGTACT49200.019.869254
CATGGGG224050.019.508384
CGTTAAC11750.019.2663331
Produced by FastQC (version 0.11.5)