FastQCFastQC Report
Thu 26 May 2016
ERR580070_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580070_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7011756
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT184120.26258757435369967No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT150940.21526704580136558No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT94540.13483070432000202No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA104400.047.4765741
GTATCAA184300.040.2643741
CAACGCA208850.033.885225
ATCAACG216400.032.8149223
GTACTAG47350.032.178391
TATCAAC232750.031.3581642
AACGCAG239150.030.3054546
GCATCGT44700.030.282078
TCAACGC241450.029.6238354
GTACATG255350.029.208321
TACATGG259150.028.3088322
ACGCAGA256850.027.4854817
ACATGGG258300.027.2369753
GTGGTAT36100.026.9651221
CGCAGAG262900.026.727068
TAGGCAT62350.026.245275
CATGGGG214300.025.9159434
CTAGGCA70250.025.0359784
ATGGGGG166650.024.661215
TCTAACG24200.024.4852052
Produced by FastQC (version 0.11.5)