FastQCFastQC Report
Thu 26 May 2016
ERR580070_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580070_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7011756
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT161850.23082662887870029No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT140860.20089118902597297No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT89260.12730049362812967No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA108650.047.0309681
GTATCAA185700.038.427661
CAACGCA216300.031.8113355
ATCAACG222400.031.0662773
GTACTAG46750.030.468081
TATCAAC240600.029.6350672
AACGCAG246150.028.4113046
TCAACGC248850.028.0471844
GTACATG252700.026.9182831
TACATGG256700.026.1834052
ACGCAGA264900.026.0277257
ACATGGG253150.025.5845583
CGCAGAG273950.025.1507388
CTGTGCG64400.024.3752279
TAGGCAT63550.024.1101235
TAGTACT66400.023.7832764
CATGGGG210500.023.5559624
GCATCGT54150.023.4343768
GTGGTAT38350.023.1675741
GGGCACC60400.023.03653794
Produced by FastQC (version 0.11.5)