FastQCFastQC Report
Thu 26 May 2016
ERR580068_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580068_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7257889
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT194090.26741935568317454No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT163510.22528589235795698No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84750.11676949041243259No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA112800.051.989571
GTATCAA206700.042.1820951
ATCAACG242300.034.7259983
CAACGCA243150.034.6046035
TATCAAC259450.033.2339252
AACGCAG265700.032.147326
TCAACGC265900.031.8036444
GTGGTAT42650.029.1101021
ACGCAGA290700.028.849927
CGCAGAG296450.028.2105128
GTACATG274700.025.833871
TACATGG275150.025.2546232
GCAGAGT331100.024.6778189
ACATGGG279200.024.1765383
GTACTAG39300.023.9329551
GCATCGT36250.022.9407548
CATGGGG217950.022.9266434
GAGTACT178250.022.55185712-13
TGGTATC52900.022.4854132
ATGGGGG166700.021.9945015
Produced by FastQC (version 0.11.5)