FastQCFastQC Report
Thu 26 May 2016
ERR580068_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580068_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7257889
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT170770.2352888009171813No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT153890.2120313496114366No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT81400.11215382323978777No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA116100.051.1097761
GTATCAA208900.041.4598771
CAACGCA244250.034.2529265
ATCAACG244000.034.2494933
TATCAAC257950.033.1559942
AACGCAG266800.031.9544076
TCAACGC267050.031.4690884
GTGGTAT42400.029.1653211
ACGCAGA296550.028.1623447
CGCAGAG302550.027.464618
GTACATG276850.026.1888661
TACATGG280650.025.4984912
GTACTAG38850.025.0527951
ACATGGG282450.024.2787283
GCAGAGT334950.024.217279
CATGGGG221200.022.4594484
TGGTATC52200.022.3380892
GAGTACT182650.021.89594712-13
ATGGGGG169850.021.6951525
TGGGGGG151450.021.2569336
Produced by FastQC (version 0.11.5)