FastQCFastQC Report
Thu 26 May 2016
ERR580067_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580067_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7531289
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT251850.33440490731400696No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT202410.26875877422842226No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT120180.15957427739129385No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA139500.051.2730561
GTATCAA259900.041.7784041
CAACGCA297750.034.921845
GTGGTAT39600.034.4379271
ATCAACG305450.034.14813
GTACTAG63700.033.0729371
TATCAAC329100.032.842012
AACGCAG337000.031.6606756
TCAACGC356200.029.4687394
TAGGCAT76900.029.2190045
ACGCAGA353400.029.2084167
CGCAGAG358600.028.6927748
GCATCGT69800.027.7992678
GTACATG358850.027.4932881
AGGGCTA79850.026.9621435
TACATGG366800.026.5954572
GGGCACC69000.026.50183194
CTAGGCA85600.026.3044034
ACATGGG360850.026.2220383
GCAGAGT385700.026.1916079
Produced by FastQC (version 0.11.5)