FastQCFastQC Report
Thu 26 May 2016
ERR580067_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580067_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7531289
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT218500.2901229789482252No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT188500.2502891603283316No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT112170.14893864781978225No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA143150.050.4156721
GTATCAA256600.040.4933931
GTGGTAT41800.033.6312871
CAACGCA300300.033.3530355
ATCAACG306300.032.715473
GTACTAG64650.032.21691
TATCAAC330400.031.2782152
GGGCACC69400.030.54104694
AACGCAG339650.030.3721496
TAGGCAT78950.029.5281855
TCAACGC359550.028.0795964
ACGCAGA363450.027.4781117
CTAGGCA84950.027.3878334
CGCAGAG367100.027.0638968
AGGGCTA85600.026.5753385
CTGTGCG88200.026.3220359
TAGTACT89050.026.2851984
GTACATG356550.025.9509451
TACATGG361800.025.2888222
GCTATTG87600.025.2689118
Produced by FastQC (version 0.11.5)