FastQCFastQC Report
Thu 26 May 2016
ERR580065_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580065_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2997101
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58120.19392072539430602No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45310.1511794230491398No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA46900.051.1460341
GTATCAA86200.041.032241
CAACGCA98200.034.755965
ATCAACG99450.034.2712633
TATCAAC105700.033.0998082
AACGCAG108800.031.9117986
TCAACGC112350.030.5041284
ACGCAGA118050.028.6570367
CGCAGAG118700.028.263088
GTGGTAT18050.027.621251
GCAGAGT131550.024.75439
GTACTAG19500.023.6377491
TGGTATC20400.023.512432
GTACATG119600.023.0059031
GCATCGT17200.022.9468358
TACATGG120650.022.3333992
CGTTAAC5350.021.9786431
GAGTACT63350.021.5860312-13
GTGATCG11600.021.4679158
ACATGGG121200.021.255673
Produced by FastQC (version 0.11.5)