FastQCFastQC Report
Thu 26 May 2016
ERR580065_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580065_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2997101
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52270.17440186366759078No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42490.14177033072959505No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA48000.048.684771
GTATCAA83450.039.4975051
CAACGCA95250.033.456155
ATCAACG95700.033.2017173
TATCAAC103750.031.494772
AACGCAG105200.030.870056
TCAACGC107900.029.7961674
ACGCAGA116700.027.0221217
CGCAGAG117750.026.8605358
CGTTAAC6050.026.4241811
GTGGTAT17700.024.1738641
GCAGAGT130950.023.7214939
GTACTAG19350.022.5985181
GTACATG119150.021.4675671
GAGTACT62000.021.03343212-13
TACATGG120400.020.9305212
ACATGGG118700.020.7890363
TGGTATC19500.020.7350732
GCTATTG25050.020.6369488
GGGCTAT27050.020.3279536
Produced by FastQC (version 0.11.5)