FastQCFastQC Report
Thu 26 May 2016
ERR580064_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580064_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9620118
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT233730.24295959779287532No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT187870.19528866485837285No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT103870.10797164858060992No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA140700.050.929331
GTATCAA256200.041.4218521
CAACGCA296150.034.6224445
ATCAACG301450.033.9706463
TATCAAC320000.032.8997422
AACGCAG327550.031.9958956
TCAACGC329100.031.3152484
ACGCAGA360800.028.1869897
GTACTAG48800.027.8455071
CGCAGAG371950.027.3036928
GTGGTAT57100.025.0330941
GTACATG350300.024.9257831
GCAGAGT410350.024.431169
TACATGG357550.024.052782
ACATGGG359550.023.2505763
TAGTACT64750.022.5094624
CTAGTAC65600.022.147053
GCATCGT53500.021.433218
CATGGGG267950.021.389134
GAGTACT214050.021.23679212-13
Produced by FastQC (version 0.11.5)