FastQCFastQC Report
Thu 26 May 2016
ERR580064_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580064_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9620118
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT209430.21770003236966534No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT176120.18307467746237624No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT103270.10734795560719734No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA139950.049.6785551
GTATCAA253800.041.9825671
CAACGCA305000.033.6998835
ATCAACG306950.033.425593
TATCAAC327450.032.1362532
AACGCAG335500.031.2086496
TCAACGC333000.031.021994
GTACTAG45700.029.1056481
ACGCAGA374000.027.3300237
CGCAGAG389800.026.1259238
GTGGTAT56350.024.1906911
GTACATG346500.024.0398541
GCAGAGT422600.023.6086189
TACATGG350650.023.2413542
ACATGGG354900.022.1423263
TAGGCAT70650.021.2206135
CTGTGCG63250.020.7297349
AGAGTAC386250.020.5617110-11
CTAGGCA73900.020.3512784
GAGTACT226550.020.24594512-13
Produced by FastQC (version 0.11.5)