FastQCFastQC Report
Thu 26 May 2016
ERR580063_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580063_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6064126
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT182500.3009502111268796No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT133890.22079026722070091No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86480.1426091740178222No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA85200.048.7395131
GTATCAA157000.041.6665531
CAACGCA183050.034.321365
ATCAACG185900.033.722923
GTACTAG38900.032.400021
TATCAAC200300.032.284922
AACGCAG205650.031.173656
GCATCGT34950.030.793948
TCAACGC206600.030.7755974
GTACATG210750.028.7860571
ACGCAGA222400.028.1918167
TACATGG212300.028.0010992
CGCAGAG225750.027.7094068
ACATGGG210250.027.2449323
TAGGCAT50100.025.6243445
GTGGTAT34800.025.5412771
CTGTGCG48900.025.469339
TAACGCC16500.025.3668274
CATGGGG163150.025.106794
TAGTACT53600.024.7425484
Produced by FastQC (version 0.11.5)