FastQCFastQC Report
Thu 26 May 2016
ERR580063_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580063_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6064126
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT158020.260581656779559No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT123030.20288166835583563No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83870.13830517373814463No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA86950.048.552931
GTATCAA156850.039.8635331
CAACGCA179800.033.276685
ATCAACG181100.033.1675723
TATCAAC197150.031.4222872
GTACTAG36400.030.7386111
AACGCAG203450.030.1469466
TCAACGC201650.030.1371044
GTACATG206800.026.9614281
ACGCAGA224450.026.8237827
TACATGG209650.026.1410392
CGCAGAG230400.026.1310678
CTGTGCG50100.025.6103469
ACATGGG208100.025.4086553
TAGGCAT48550.024.8796985
CTAACGC14900.023.6579043
TAGTACT54650.023.6506884
CATGGGG164850.023.4360354
GTGGTAT34000.023.367731
GCATCGT44500.023.2355618
Produced by FastQC (version 0.11.5)