FastQCFastQC Report
Thu 26 May 2016
ERR580062_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580062_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6325943
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT91250.14424726874712593No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75820.11985564839898177No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA94750.046.5530321
GTATCAA169650.040.3582081
CAACGCA187550.034.9967275
ATCAACG192750.034.0810243
TATCAAC207750.032.5948752
AACGCAG211750.031.6043196
TCAACGC217200.030.3737344
ACGCAGA233450.027.7580577
CGCAGAG236300.027.164538
GTACTAG41150.027.0833821
GCATCGT40200.024.4306748
GCAGAGT263250.023.7980989
GTACATG280400.023.7135071
TACATGG281600.023.3955522
ACATGGG281500.022.3672623
GTGGTAT41000.021.9065441
CTGTGCG53650.021.9003339
GTATAAG43050.021.6280021
TAGTACT59000.021.5982024
TAGGCAT58650.021.08375
Produced by FastQC (version 0.11.5)