FastQCFastQC Report
Thu 26 May 2016
ERR580062_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580062_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6325943
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84850.13413020003499873No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70880.11204653598680861No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA99550.043.1598051
GTATCAA174550.038.233051
CAACGCA190000.033.491695
ATCAACG195800.032.548123
TATCAAC212050.031.0948142
AACGCAG216750.030.1160476
TCAACGC220450.029.1216534
GTACTAG43000.027.3107261
ACGCAGA236250.026.8942667
CGCAGAG240100.026.2672848
GTACATG285150.023.8172421
TACATGG285550.023.5025312
GCAGAGT267550.022.8170269
ACATGGG285350.022.6466353
TAGGCAT58050.022.2639645
GTATAAG40300.021.5335241
GCATCGT50300.021.4889248
GTGATCG22350.021.447548
GTGGTAT44500.021.19691
CTGTGCG52350.021.0962879
Produced by FastQC (version 0.11.5)