##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR580062_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6325943
Sequences flagged as poor quality	0
Sequence length	100
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.54805283575903	34.0	31.0	34.0	31.0	34.0
2	32.74743939362084	34.0	31.0	34.0	31.0	34.0
3	32.83200828714391	34.0	31.0	34.0	31.0	34.0
4	36.29057501782738	37.0	37.0	37.0	35.0	37.0
5	36.229365487485424	37.0	37.0	37.0	35.0	37.0
6	36.25883318898068	37.0	37.0	37.0	35.0	37.0
7	36.23062174287691	37.0	37.0	37.0	35.0	37.0
8	36.222766155180345	37.0	37.0	37.0	35.0	37.0
9	38.01056095510187	39.0	38.0	39.0	35.0	39.0
10-11	37.96365585336447	39.0	38.0	39.0	35.0	39.0
12-13	37.9268786487643	39.0	38.0	39.0	35.0	39.0
14-15	39.33385567969866	41.0	39.0	41.0	36.0	41.0
16-17	39.32189185074857	41.0	39.0	41.0	36.0	41.0
18-19	39.320709181224046	41.0	39.0	41.0	36.0	41.0
20-21	39.304965915753584	41.0	39.0	41.0	36.0	41.0
22-23	39.23140146852414	41.0	39.0	41.0	36.0	41.0
24-25	39.14990895428555	41.0	39.0	41.0	35.5	41.0
26-27	39.01674643606495	40.0	38.5	41.0	35.0	41.0
28-29	38.91681327195013	40.0	38.0	41.0	35.0	41.0
30-31	38.821144768455866	40.0	38.0	41.0	35.0	41.0
32-33	38.695502393872346	40.0	38.0	41.0	35.0	41.0
34-35	38.669685294350586	40.0	38.0	41.0	35.0	41.0
36-37	38.7227250545887	40.0	38.0	41.0	35.0	41.0
38-39	38.63456950212799	40.0	38.0	41.0	35.0	41.0
40-41	38.520331909408604	40.0	38.0	41.0	34.0	41.0
42-43	38.500419384113954	40.0	38.0	41.0	34.0	41.0
44-45	38.41867370603877	40.0	38.0	41.0	34.0	41.0
46-47	38.24067700262238	40.0	38.0	41.0	34.0	41.0
48-49	38.04550681218595	40.0	37.0	41.0	33.0	41.0
50-51	37.87493437737267	40.0	37.0	41.0	33.0	41.0
52-53	37.69726339614505	40.0	36.5	41.0	33.0	41.0
54-55	37.49653055678813	39.5	36.0	41.0	33.0	41.0
56-57	37.27893430275928	39.0	35.5	41.0	32.5	41.0
58-59	37.039719611763815	39.0	35.0	41.0	32.0	41.0
60-61	36.77818200385302	38.5	35.0	40.5	32.0	41.0
62-63	36.48588581971099	38.0	35.0	40.0	31.0	41.0
64-65	36.19618529601041	37.0	35.0	40.0	31.0	41.0
66-67	35.86833939224555	37.0	35.0	40.0	31.0	41.0
68-69	35.514246097380266	36.0	35.0	39.0	31.0	41.0
70-71	35.192594843171996	36.0	34.5	39.0	30.0	40.5
72-73	34.845702292922965	35.0	34.0	38.5	30.0	40.0
74-75	34.492537792389214	35.0	34.0	37.0	30.0	39.0
76-77	33.26582250266877	34.5	32.5	36.0	28.0	39.0
78-79	33.89196132497558	35.0	34.0	36.0	29.5	39.0
80-81	33.82522455545363	35.0	34.0	36.0	30.0	37.5
82-83	33.62354782520171	35.0	34.0	35.5	29.5	37.0
84-85	33.41685366118537	35.0	34.0	35.0	29.0	37.0
86-87	33.25722093923388	35.0	34.0	35.0	29.0	36.0
88-89	33.107064511962875	35.0	34.0	35.0	29.0	36.0
90-91	32.95797835990618	35.0	33.0	35.0	29.0	36.0
92-93	32.850522839045496	35.0	33.0	35.0	29.0	35.0
94-95	32.761710625593686	35.0	33.0	35.0	29.0	35.0
96-97	32.67300266537337	35.0	33.0	35.0	29.0	35.0
98-99	32.606052172774874	35.0	33.0	35.0	29.0	35.0
100	31.32915630128188	34.0	31.0	35.0	24.0	35.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.195050655425284
1101	2	0.13004845441255952
1101	3	0.07709359022687323
1101	4	0.046686253138908285
1101	5	0.06663780434136157
1101	6	0.05747373246951071
1101	7	0.026322033369723385
1101	8	0.03202782718044972
1101	9	0.0031870062940626553
1101	10-11	0.015049052987656353
1101	12-13	-0.0017469438490778089
1101	14-15	0.07375476919922619
1101	16-17	0.019136997967784453
1101	18-19	0.02304992472586065
1101	20-21	-0.0175579213688124
1101	22-23	0.007930212363035594
1101	24-25	-0.058956948239739404
1101	26-27	-0.06762213686584317
1101	28-29	-0.0487642738158911
1101	30-31	0.007955477390098054
1101	32-33	-0.004660246506155374
1101	34-35	-0.0654132070918294
1101	36-37	0.004820940219616432
1101	38-39	0.021279975315273703
1101	40-41	0.029926091506418118
1101	42-43	0.01906847289516378
1101	44-45	-0.047287463825888665
1101	46-47	0.07596341110257043
1101	48-49	0.05924186379318286
1101	50-51	0.056810265254846115
1101	52-53	0.012985308584255506
1101	54-55	0.03452706253746385
1101	56-57	-0.0025971725811473334
1101	58-59	-0.03429321094119331
1101	60-61	-0.05915981468142917
1101	62-63	-0.10064170796328398
1101	64-65	-0.08190751949728536
1101	66-67	-0.11491933198137616
1101	68-69	-0.08967413455661699
1101	70-71	-0.10140942870356895
1101	72-73	-0.03927370606366054
1101	74-75	-0.08956223990115575
1101	76-77	-0.06817817250706781
1101	78-79	-0.03169795159438138
1101	80-81	-0.04111611935154258
1101	82-83	-0.010859621643540152
1101	84-85	-0.03243154583424257
1101	86-87	0.03711247316069688
1101	88-89	0.02481319299941731
1101	90-91	0.061890518496106495
1101	92-93	-0.08449224823247903
1101	94-95	-0.11601789119257688
1101	96-97	-0.049334830002315755
1101	98-99	-0.11900273556740615
1101	100	0.07897569371453983
1102	1	0.08091840455400501
1102	2	0.04233492211998424
1102	3	-0.01016085920124965
1102	4	-0.02709281398270491
1102	5	-0.009230639912821914
1102	6	0.035582531107664295
1102	7	0.028254232131601498
1102	8	0.016675356059415947
1102	9	0.02051508401209645
1102	10-11	0.020101589970579425
1102	12-13	-0.015631525015983527
1102	14-15	0.00971918577641162
1102	16-17	-0.06542981634219558
1102	18-19	5.582899857969892E-4
1102	20-21	-0.02888449081459754
1102	22-23	-0.04292974285888107
1102	24-25	-0.02876634883654816
1102	26-27	-0.0714311994016299
1102	28-29	-0.0591131667910787
1102	30-31	-0.04725276269724077
1102	32-33	-0.030450451638181164
1102	34-35	-0.057181471263511696
1102	36-37	-0.0317401090497782
1102	38-39	-0.011396097715902442
1102	40-41	-0.0276254715507136
1102	42-43	-0.019604632600376704
1102	44-45	-0.05839269331981711
1102	46-47	0.0014064285672148458
1102	48-49	0.008718644352079252
1102	50-51	0.01619355893935648
1102	52-53	-0.052324079760332154
1102	54-55	-0.11414572290031089
1102	56-57	-0.08711937883938248
1102	58-59	-0.059719575399981295
1102	60-61	-0.0346795209884192
1102	62-63	-0.05797298125811068
1102	64-65	-0.09177292520301705
1102	66-67	-0.10486119148701789
1102	68-69	-0.07670884432172898
1102	70-71	-0.11021996900609565
1102	72-73	-0.10003592364393654
1102	74-75	-0.1557834928373083
1102	76-77	-0.1382382933588886
1102	78-79	-0.09336438238513978
1102	80-81	-0.12909649981021687
1102	82-83	-0.10942601287715803
1102	84-85	-0.060215660867001475
1102	86-87	-0.07669234360873389
1102	88-89	-0.017061515385449866
1102	90-91	-0.02211271908828394
1102	92-93	-0.01412017107254826
1102	94-95	-0.13360371543385696
1102	96-97	-0.14045215167887903
1102	98-99	-0.10992907277127273
1102	100	-0.06957216917471243
1103	1	0.1476604077296173
1103	2	0.05514273294477334
1103	3	0.044992011970798274
1103	4	0.021560168230124077
1103	5	0.03343092441755857
1103	6	0.04046278584175411
1103	7	0.03399632444199341
1103	8	0.006002700744588196
1103	9	-0.010391869859908809
1103	10-11	0.010207630135617762
1103	12-13	-0.007437823571144975
1103	14-15	0.008131957329027273
1103	16-17	0.029423833928198917
1103	18-19	0.003038930869848855
1103	20-21	-0.06401207691903466
1103	22-23	-0.06462617902906942
1103	24-25	-0.05459838669414552
1103	26-27	-0.08166923456907682
1103	28-29	-0.02468956914040632
1103	30-31	-0.09176543469084208
1103	32-33	-0.10169366014758197
1103	34-35	-0.08247333728739648
1103	36-37	-0.06526173329685037
1103	38-39	-0.045803102198327394
1103	40-41	-0.04711222144004523
1103	42-43	-0.06986657300900134
1103	44-45	-0.06136694948509813
1103	46-47	-0.1527918696132744
1103	48-49	-0.11091603400988248
1103	50-51	-0.11880382492987707
1103	52-53	-0.11105855734243875
1103	54-55	-0.08282683544328506
1103	56-57	-0.06105843583376469
1103	58-59	-0.0896485143386343
1103	60-61	-0.025177854873184913
1103	62-63	-0.13053102839077013
1103	64-65	-0.09031139189355031
1103	66-67	-0.0875205501094456
1103	68-69	-0.06130424043515603
1103	70-71	-0.10454256235463077
1103	72-73	-0.05365260483906553
1103	74-75	-0.061994423983463776
1103	76-77	-0.13530271262066407
1103	78-79	-0.037332954571965615
1103	80-81	-0.06219556480155575
1103	82-83	-0.00914581746553722
1103	84-85	-0.05701267580890601
1103	86-87	-0.0054380813802765715
1103	88-89	0.023291936723751405
1103	90-91	0.025854731108289286
1103	92-93	0.030754151415038677
1103	94-95	-0.0212707864803221
1103	96-97	0.04171666353869341
1103	98-99	0.025994834333168626
1103	100	0.038830722014893126
1104	1	0.14337462270718504
1104	2	0.07452716499889078
1104	3	0.05068817676382764
1104	4	0.025215631708277897
1104	5	0.019287722321656986
1104	6	5.221987401355932E-4
1104	7	-0.007487204746027487
1104	8	-0.006901865242824101
1104	9	0.0030486701794885107
1104	10-11	0.007969423609324622
1104	12-13	-0.02498738648723986
1104	14-15	-0.018947445788136008
1104	16-17	-0.028041892802505686
1104	18-19	-0.048619183557228496
1104	20-21	-0.03220987178989532
1104	22-23	-0.06641677398926049
1104	24-25	-0.05630913200365484
1104	26-27	-0.018331473074482574
1104	28-29	-0.05735714671760661
1104	30-31	-0.08435755806554113
1104	32-33	-0.12550309367750145
1104	34-35	-0.07199110041467094
1104	36-37	-0.02644164348160416
1104	38-39	0.004785331735909892
1104	40-41	0.00956753640584651
1104	42-43	-0.03875907471044826
1104	44-45	-0.10284542301553046
1104	46-47	-0.089858263333916
1104	48-49	-0.09897665253389931
1104	50-51	-0.1351768694126676
1104	52-53	-0.15366206338149624
1104	54-55	-0.17063290160786693
1104	56-57	-0.1329954619680933
1104	58-59	-0.07370289596553903
1104	60-61	-0.13544531551600159
1104	62-63	-0.05259925627424167
1104	64-65	-0.05858375771379798
1104	66-67	-0.11541279887931921
1104	68-69	-0.13370672163294728
1104	70-71	-0.1291385595526009
1104	72-73	-0.11990019466583846
1104	74-75	-0.16046439612827612
1104	76-77	-0.21669359539646393
1104	78-79	-0.13375241147836192
1104	80-81	-0.11558966565210937
1104	82-83	-0.06638006199192859
1104	84-85	0.04191721248038505
1104	86-87	-0.04023554058520773
1104	88-89	-0.05533506265871324
1104	90-91	-0.06213787395162029
1104	92-93	-0.18302261959296118
1104	94-95	-0.14877655253214073
1104	96-97	-0.13454065991588493
1104	98-99	-0.12389444676570349
1104	100	-0.07410605148525207
1105	1	0.0786217023156226
1105	2	0.06448448210721125
1105	3	0.06099123149350305
1105	4	0.011653575403173022
1105	5	0.014975270037901112
1105	6	-0.01239627240949659
1105	7	-0.02163457308380856
1105	8	-0.0024286553583010573
1105	9	0.039746892851653115
1105	10-11	-0.0037619135608224497
1105	12-13	8.398717645050624E-4
1105	14-15	-0.028716985476542334
1105	16-17	-0.010587862421317595
1105	18-19	-0.027533997151856227
1105	20-21	-8.56567996883939E-4
1105	22-23	0.007886004136040015
1105	24-25	-0.031205319134862464
1105	26-27	-0.039081420186079185
1105	28-29	-0.029742452078785675
1105	30-31	-0.0667454473669622
1105	32-33	-0.041733248585842375
1105	34-35	-0.0528193274329638
1105	36-37	-0.014125090757467262
1105	38-39	0.023696069588687863
1105	40-41	-0.02044007069688547
1105	42-43	0.08035224955423104
1105	44-45	0.07339692149164989
1105	46-47	0.07732824252112636
1105	48-49	0.06322580088517071
1105	50-51	0.07061161261034954
1105	52-53	0.044967399080057646
1105	54-55	0.020373671798168402
1105	56-57	0.001529440952047878
1105	58-59	0.011667084215567058
1105	60-61	0.07289405074980237
1105	62-63	0.04262732354425225
1105	64-65	0.04025577983783535
1105	66-67	0.00490479832301105
1105	68-69	0.022910544813939282
1105	70-71	0.047874251634247855
1105	72-73	-0.004029733984722839
1105	74-75	-0.02907693164660685
1105	76-77	-0.01358928291282524
1105	78-79	0.04653913117400066
1105	80-81	0.06702714584955771
1105	82-83	0.09567606944888496
1105	84-85	0.11458954915084973
1105	86-87	0.06383872513304567
1105	88-89	0.0875359938023692
1105	90-91	0.134176715238425
1105	92-93	0.058267327374394995
1105	94-95	0.0366676390497247
1105	96-97	0.023750994024645422
1105	98-99	-0.05133154103818072
1105	100	0.08396517279084748
1106	1	0.08284540534639717
1106	2	0.04765608891871409
1106	3	-0.03295280650711163
1106	4	-0.0034596919541769466
1106	5	-0.018879758375582867
1106	6	-0.013250174914524848
1106	7	-0.015072580467446528
1106	8	-0.01856260947148769
1106	9	-0.0047431865901259584
1106	10-11	0.009559915049820233
1106	12-13	-0.03203195587969532
1106	14-15	-0.027467323732302873
1106	16-17	-0.06012511646105878
1106	18-19	-0.03776381777965554
1106	20-21	-0.07908593197941371
1106	22-23	-0.07207853941197584
1106	24-25	-0.031072831601996143
1106	26-27	-0.09060661540796389
1106	28-29	-0.06518339262308359
1106	30-31	-0.05942416721388355
1106	32-33	-0.040075179253079796
1106	34-35	-0.06635304911612394
1106	36-37	0.007563204143565372
1106	38-39	-0.03974383444332119
1106	40-41	-0.04116585883280521
1106	42-43	-0.03625132435949041
1106	44-45	-0.056597799302380736
1106	46-47	-0.10505677468159291
1106	48-49	-0.10819288001400906
1106	50-51	-0.07600743650439057
1106	52-53	-0.08147708590138336
1106	54-55	-0.11327557630760055
1106	56-57	-0.1963428336729791
1106	58-59	-0.19048572040789935
1106	60-61	-0.19520992907082757
1106	62-63	-0.23638869044987842
1106	64-65	-0.1829535908131561
1106	66-67	-0.16607974338875664
1106	68-69	-0.16797927320446604
1106	70-71	-0.14067918552748893
1106	72-73	-0.1672458335092344
1106	74-75	-0.1711522032176589
1106	76-77	-0.158256741259045
1106	78-79	-0.1655866733914806
1106	80-81	-0.06828305884349817
1106	82-83	-0.05925725792470615
1106	84-85	-0.07737740809362492
1106	86-87	0.008740377270818556
1106	88-89	-0.024694488840282247
1106	90-91	-0.023652190165940112
1106	92-93	-0.014525529630397216
1106	94-95	-0.0013196425337014261
1106	96-97	-0.041109837891234235
1106	98-99	0.004207095017946472
1106	100	0.001346759113719287
1107	1	0.0678359121659895
1107	2	0.014136734454979205
1107	3	-0.04124393716427477
1107	4	-0.0028319796169213873
1107	5	-0.025840681345201233
1107	6	-0.0239117914675262
1107	7	-0.046412378204578886
1107	8	-0.04174970262090483
1107	9	-0.03562033708609391
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2316	9	5.276392545923159E-4
2316	10-11	0.0014083491799397052
2316	12-13	-0.014179298169409549
2316	14-15	0.04952921652650133
2316	16-17	0.05978833384447313
2316	18-19	0.031525362666066314
2316	20-21	0.009338930978891824
2316	22-23	0.004715864344319698
2316	24-25	-0.023967500455341906
2316	26-27	0.010067135490658075
2316	28-29	0.05077118142436632
2316	30-31	-0.2306237313763475
2316	32-33	-0.09128738045725271
2316	34-35	-0.11161516944322614
2316	36-37	-0.4365622564063969
2316	38-39	-0.2541422550939316
2316	40-41	-0.22496107135976473
2316	42-43	-0.24231418381312153
2316	44-45	-0.10792648594868126
2316	46-47	-0.2447875214465185
2316	48-49	-0.2623944998019354
2316	50-51	-0.3702961114684413
2316	52-53	-0.3185691621954163
2316	54-55	-0.20548374489135313
2316	56-57	-0.9633306122495426
2316	58-59	-0.6849494605229296
2316	60-61	-0.3532026052031014
2316	62-63	-0.1853514420674145
2316	64-65	-0.12289967276858249
2316	66-67	-0.10981794881651297
2316	68-69	-0.17964730654378513
2316	70-71	-0.15079932167055432
2316	72-73	-0.07389566950850934
2316	74-75	-0.1045246645763882
2316	76-77	-0.0954891167982268
2316	78-79	-0.10176540047631022
2316	80-81	-0.14281902667239876
2316	82-83	-0.3056291913359175
2316	84-85	-0.14147405618852105
2316	86-87	-0.1515165759103425
2316	88-89	-0.16340932978204137
2316	90-91	-0.13454172557211308
2316	92-93	-0.17361105712859626
2316	94-95	-0.13634570292190773
2316	96-97	-0.30191860855982355
2316	98-99	-0.10154725212439075
2316	100	-0.4025487072773686
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	6.0
13	38.0
14	193.0
15	575.0
16	1416.0
17	2921.0
18	5421.0
19	8575.0
20	13086.0
21	19466.0
22	26245.0
23	28036.0
24	27910.0
25	30493.0
26	36403.0
27	45806.0
28	56928.0
29	71689.0
30	88502.0
31	110871.0
32	140412.0
33	185266.0
34	276772.0
35	431112.0
36	781942.0
37	1652535.0
38	1932073.0
39	351187.0
40	64.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.719357959909615	20.108347755428134	13.765714319256237	23.40657996540601
2	15.700121073432404	20.619363560643343	39.500543241742335	24.179972124181916
3	19.047610767115142	26.106762177913147	29.793985504164915	25.051641550806796
4	13.069890796273025	17.413505750896753	39.242284436813016	30.27431901601721
5	13.691656762508565	38.33196904148301	34.22244691671622	13.7539272792922
6	30.05942355155587	37.457672318577636	18.48560760032141	13.99729652954508
7	27.404094064785333	32.58495419967758	22.106776892217407	17.90417484331968
8	26.814158186876448	34.113061445736854	20.052868174335984	19.019912193050722
9	26.320863958746493	16.034044894174855	20.793896074229757	36.851195072848896
10-11	25.515316736747167	25.897844897188786	27.697182371092865	20.889655994971182
12-13	26.288270788488227	23.32646637077393	27.69664623808537	22.688616602652477
14-15	23.6768406808812	24.825717049852038	25.39372929154969	26.103712977717073
16-17	22.985303912757253	27.640196457899393	26.072689169846896	23.301810459496465
18-19	22.455012957096702	27.338920753596696	27.92182016817589	22.284246121130714
20-21	23.95335893063578	26.41306957857718	27.506079346157485	22.127492144629553
22-23	23.9460762461563	26.094803105692566	27.1591996246561	22.79992102349504
24-25	23.446132853236268	26.256828428583688	27.545363592431986	22.75167512574805
26-27	23.223690643033525	26.69429213402151	27.011002788498633	23.071014434446333
28-29	22.799778347455383	26.68118000731991	27.504005792688467	23.01503585253624
30-31	23.527070252489597	26.284650963383495	27.489524522560732	22.698754261566183
32-33	23.450909339559363	26.57016161513156	26.92425325777671	23.054675787532368
34-35	23.525404950173144	26.6823298070188	27.249034366764906	22.54323087604315
36-37	23.448501834540746	26.368186379695985	26.931848678747272	23.251463107015997
38-39	23.35586877083844	26.497296743523354	27.261452655107416	22.88538183053079
40-41	23.495646919452636	25.856137988234423	27.6024768772981	23.045738215014843
42-43	23.272510173422045	25.914011285340994	27.32142227609895	23.492056265138007
44-45	23.47828870563742	26.281846485573883	27.156668940404106	23.08319586838459
46-47	23.398693306420405	26.17983572742058	26.66775115151423	23.753719814644782
48-49	23.701911618522125	26.159668465705295	27.013460181695653	23.124959734076928
50-51	23.632076094342683	26.182581558023415	26.7710198978726	23.414322449761304
52-53	23.350229812991003	26.379336402143807	26.667539314193895	23.602894470671295
54-55	23.27290378482494	26.199557575775778	26.842452806475524	23.685085832923754
56-57	23.709693571513878	26.197976123508802	26.479073154387827	23.61325715058949
58-59	23.06681750663679	26.415537560251217	27.077426573996988	23.440218359115
60-61	23.359098780034	26.57713177890183	26.683938479087765	23.379830961976403
62-63	23.424526143190295	26.268896078926534	26.579390306411792	23.72718747147137
64-65	22.95038637833777	26.152305971428046	26.90923887166523	23.988068778568948
66-67	23.321611355535246	26.284019746543812	26.702484157703925	23.691884740217013
68-69	23.066421587322164	26.17820472134739	26.91571913148328	23.83965455984717
70-71	23.083294326982767	25.903544369150726	26.790217602479206	24.222943701387297
72-73	23.081500805118356	26.24575190593157	26.766043639217184	23.906703649732886
74-75	23.221436541509668	26.36715347322764	26.682514834536462	23.728895150726224
76-77	22.956052418808873	26.364920661431256	26.38060270421968	24.298424215540194
78-79	22.981946452080418	26.251618755303635	26.54596664352352	24.220468149092422
80-81	22.87241698495388	26.790845247483112	26.49581289920599	23.840924868357018
82-83	23.32387675202514	26.251746877672282	27.01681231192981	23.407564058372763
84-85	23.260468347756944	26.332693367141673	26.51863661117746	23.88820167392392
86-87	22.551608271551217	26.716234636478504	26.878763195762655	23.853393896207628
88-89	22.959155767188474	26.07224528987306	26.829702246564015	24.138896696374456
90-91	22.95802110314715	26.287556048096338	26.62048741145272	24.133935437303787
92-93	22.932946516307144	26.28635578398107	26.828443950946408	23.952253748765376
94-95	22.442062270861626	26.251706224517292	27.099259841009793	24.20697166361129
96-97	22.54812527886756	26.246680759729095	26.939343317038972	24.265850644364377
98-99	22.25663764378782	26.402056715546806	26.85877264175014	24.48253299891524
100	22.55456291414912	26.175180859376667	26.47370573484244	24.796550491631773
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	87.0
1	71.5
2	105.5
3	171.5
4	130.5
5	114.0
6	260.5
7	523.5
8	861.5
9	1033.5
10	944.0
11	870.0
12	960.5
13	1136.0
14	1340.0
15	1595.5
16	1924.5
17	2307.5
18	2723.0
19	3124.0
20	3565.5
21	4105.5
22	4866.5
23	5859.0
24	6950.0
25	8303.0
26	10303.0
27	12658.5
28	15475.0
29	19428.0
30	25095.5
31	32334.0
32	43116.5
33	57662.0
34	73418.0
35	91445.5
36	115904.0
37	145160.0
38	169628.5
39	189846.5
40	201627.5
41	209543.5
42	220896.0
43	236991.5
44	256928.5
45	283317.5
46	304210.5
47	318296.0
48	328861.0
49	324952.5
50	312552.5
51	300447.0
52	287113.0
53	270262.5
54	254679.5
55	227769.0
56	197950.0
57	163742.0
58	130653.5
59	114269.0
60	92150.0
61	66640.0
62	49943.5
63	38366.0
64	26895.0
65	15799.0
66	10297.5
67	7269.5
68	5249.0
69	3782.5
70	2736.5
71	2019.5
72	1416.0
73	987.0
74	719.5
75	475.0
76	303.5
77	207.0
78	122.5
79	56.5
80	31.0
81	16.0
82	12.5
83	9.5
84	3.5
85	1.5
86	2.0
87	2.0
88	0.5
89	0.5
90	0.5
91	0.0
92	0.5
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.3562472820257786
2	4.7423759588096194E-4
3	0.00199179790270004
4	9.484751917619239E-4
5	0.0050111105964754975
6	0.0
7	7.903959931349365E-5
8	3.161583972539746E-4
9	0.0028138097355603743
10-11	5.532771951944556E-5
12-13	3.161583972539746E-5
14-15	3.635821568420708E-4
16-17	1.1855939897024049E-4
18-19	0.0010433227109381163
20-21	2.529267178031797E-4
22-23	1.7388711848968604E-4
24-25	0.0
26-27	0.0021735889811210756
28-29	0.011508165660044676
30-31	0.0034303186102056242
32-33	0.013997913038419726
34-35	0.010899560745330775
36-37	0.012496160651463348
38-39	0.012551488370982793
40-41	0.01394258531890028
42-43	0.006196704586177903
44-45	0.0013910969479174883
46-47	0.005675043230708845
48-49	0.012733279449403829
50-51	0.0034224146502742753
52-53	0.005769890749885037
54-55	0.005374692753317569
56-57	2.1340691814643285E-4
58-59	5.295653154004075E-4
60-61	4.584296760182632E-4
62-63	9.563791516932732E-4
64-65	3.003504773912759E-4
66-67	0.0012093058694964528
68-69	0.0015886959462012224
70-71	7.903959931349365E-4
72-73	0.002608306777345291
74-75	0.007919767851212064
76-77	0.00373857304752825
78-79	0.0015017523869563793
80-81	4.1890987636151637E-4
82-83	2.1340691814643285E-4
84-85	0.0013910969479174883
86-87	0.004513161120800488
88-89	0.0016598315855833668
90-91	2.292148380091316E-4
92-93	0.002007605822562739
94-95	0.0032801433715099865
96-97	0.0021419731413956783
98-99	0.002039221662288136
100	0.002718962216384182
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	6325943.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.22238483622905
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.31637593179342	42.82748245394082
2	15.692192378266888	18.58658111899724
3	5.634091678319841	10.009930367281594
4	2.5031969451724647	5.9298117123150655
5	1.3088671174065174	3.8757116063267274
6	0.7441450387494883	2.644202631527566
7	0.4790425968585162	1.98590315168711
8	0.29339704192763955	1.3900538021479918
9	0.21727281137119983	1.158067264452714
>10	0.7626021410066174	7.293878862723847
>50	0.030299386705368404	1.24452559563954
>100	0.016954539007535675	1.9394814765189814
>500	0.0011066974545049975	0.4382969951302483
>1k	3.905965371670609E-4	0.3758766729246126
>5k	6.509942285754215E-5	0.3001962883858232
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8485	0.13413020003499873	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7088	0.11204653598680861	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	3.161583972539746E-5	0.0
4	0.0	0.0	0.0	3.161583972539746E-5	0.0
5	0.0	0.0	0.0	3.161583972539746E-5	0.0
6	0.0	0.0	0.0	3.161583972539746E-5	3.161583972539746E-5
7	0.0	0.0	0.0	3.161583972539746E-5	3.161583972539746E-5
8	0.0	0.0	0.0	3.161583972539746E-5	3.161583972539746E-5
9	0.0	0.0	0.0	9.484751917619239E-5	3.161583972539746E-5
10-11	0.0	0.0	0.0	1.8969503835238478E-4	3.161583972539746E-5
12-13	0.0	1.580791986269873E-5	0.0	2.4502275787183036E-4	3.161583972539746E-5
14-15	0.0	5.5327719519445565E-5	0.0	2.8454255752857717E-4	4.7423759588096194E-5
16-17	0.0	7.903959931349366E-5	0.0	3.24062357185324E-4	4.7423759588096194E-5
18-19	0.0	7.903959931349366E-5	0.0	3.635821568420708E-4	6.323167945079492E-5
20-21	0.0	7.903959931349366E-5	0.0	5.374692753317569E-4	6.323167945079492E-5
22-23	0.0	7.903959931349366E-5	0.0	7.271643136841416E-4	8.694355924484303E-5
24-25	0.0	7.903959931349366E-5	0.0	0.0010275147910754176	1.343673188329392E-4
26-27	0.0	7.903959931349366E-5	0.0	0.0015175603068190782	1.4227127876428858E-4
28-29	0.0	7.903959931349366E-5	0.0	0.002379091939336159	1.5017523869563797E-4
30-31	0.0	7.903959931349366E-5	0.0	0.0060386253875509156	1.7388711848968606E-4
32-33	0.0	7.903959931349366E-5	0.0	0.015768400063041983	1.8969503835238478E-4
34-35	0.0	1.0275147910754175E-4	0.0	0.03314130399214789	2.0550295821508352E-4
36-37	0.0	1.3436731883293923E-4	0.0	0.06282067353436477	2.2131087807778223E-4
38-39	0.0	1.7388711848968606E-4	0.0	0.11093207763648835	2.3711879794048097E-4
40-41	0.0	1.9759899828373416E-4	0.0	0.16723198422748986	2.3711879794048097E-4
42-43	0.0	2.2131087807778223E-4	0.0	0.22587936691810218	2.3711879794048097E-4
44-45	0.0	2.2131087807778223E-4	0.0	0.3004137090707267	2.450227578718303E-4
46-47	0.0	2.2131087807778223E-4	0.0	0.3893727780980638	2.6873463766587845E-4
48-49	0.0	2.3711879794048097E-4	0.0	0.48794306240192176	2.8454255752857717E-4
50-51	0.0	2.529267178031797E-4	0.0	0.595255126389852	2.8454255752857717E-4
52-53	0.0	2.529267178031797E-4	0.0	0.7052703446743038	3.161583972539746E-4
54-55	0.0	2.924465174599265E-4	0.0	0.8273786216537202	3.477742369793721E-4
56-57	0.0	3.003504773912759E-4	0.0	0.9706141835296334	3.5567819691072146E-4
58-59	0.0	3.0825443732262526E-4	0.0	1.1399565250587935	3.714861167734202E-4
60-61	0.0	3.3196631711667336E-4	0.0	1.3166653572439713	3.7939007670476955E-4
62-63	0.0	3.3196631711667336E-4	0.0	1.50210806515329	3.9519799656746827E-4
64-65	0.0	3.3196631711667336E-4	0.0	1.6944193142429516	3.9519799656746827E-4
66-67	0.0	3.6358215684207084E-4	0.0	1.9095967194140067	4.2681383629286575E-4
68-69	0.0	3.6358215684207084E-4	0.0	2.15504629112213	4.663336359496126E-4
70-71	0.0	3.6358215684207084E-4	0.0	2.427740812713614	4.9794947567501E-4
72-73	0.0	3.6358215684207084E-4	0.0	2.708402525915899	5.848930349198531E-4
74-75	0.0	3.6358215684207084E-4	0.0	2.987396503572669	5.848930349198531E-4
76-77	0.0	4.1100591643016704E-4	0.0	3.2961726022507634	5.927969948512024E-4
78-79	0.0	4.1100591643016704E-4	0.0	3.641378052252447	6.007009547825518E-4
80-81	0.0	4.189098763615164E-4	0.0	4.012555914588544	6.165088746452505E-4
82-83	0.0	4.2681383629286575E-4	0.0	4.395850547499401	6.323167945079493E-4
84-85	0.0	4.2681383629286575E-4	0.0	4.781793006987259	6.402207544392986E-4
86-87	0.0	4.2681383629286575E-4	0.0	5.182294560668662	6.48124714370648E-4
88	0.0	4.2681383629286575E-4	0.0	5.50368537939719	6.48124714370648E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	9955	0.0	43.159805	1
GTATCAA	17455	0.0	38.23305	1
CAACGCA	19000	0.0	33.49169	5
ATCAACG	19580	0.0	32.54812	3
TATCAAC	21205	0.0	31.094814	2
AACGCAG	21675	0.0	30.116047	6
TCAACGC	22045	0.0	29.121653	4
GTACTAG	4300	0.0	27.310726	1
ACGCAGA	23625	0.0	26.894266	7
CGCAGAG	24010	0.0	26.267284	8
GTACATG	28515	0.0	23.817242	1
TACATGG	28555	0.0	23.502531	2
GCAGAGT	26755	0.0	22.817026	9
ACATGGG	28535	0.0	22.646635	3
TAGGCAT	5805	0.0	22.263964	5
GTATAAG	4030	0.0	21.533524	1
GCATCGT	5030	0.0	21.488924	8
GTGATCG	2235	0.0	21.44754	8
GTGGTAT	4450	0.0	21.1969	1
CTGTGCG	5235	0.0	21.096287	9
>>END_MODULE